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A. Cheraghali (2007)
Availability of blood components and plasma derived medicines in Iran.Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis, 37 1
S. Kelly, N. Price (2000)
The use of circular dichroism in the investigation of protein structure and function.Current protein & peptide science, 1 4
K. Chidwick, P. Matejtschuk, E. Gascoigne, N. Briggs, J. More, C. Dash (1999)
Clinical Experience with a New Solvent Detergent–Treated Intravenous Immunoglobulin Free of Hypotensive EffectsVox Sanguinis, 77
G. Li, R. Stewart, B. Conlan, A. Gilbert, P. Roeth, H. Nair (2002)
Purification of human immunoglobulin G: a new approach to plasma fractionationVox Sanguinis, 83
A. Sisti, M. Vitali, M. Manfredi, J. Zarzur (2001)
Preparation of lyophilized and liquid intravenous immunoglobulin G: development and scale‐upVox Sanguinis, 80
A. Aghaie, A. Pourfatollah, S. Bathaie, S. Moazzeni, H. Pour, Z. Sharifi (2008)
Inactivation of virus in intravenous immunoglobulin G using solvent/detergent treatment and pasteurization.Human antibodies, 17 3-4
A. Aghaie, A. Pourfathollah, S. Bathaie, S. Moazzeni, H. Pour, S. Banazadeh (2008)
Structural study on immunoglobulin G solution after pasteurization with and without stabilizerTransfusion Medicine, 18
V. Baikar, M. Kamath, C. Vishwanathan, A. Varadkar, S. Bharmal (2008)
Separation of antihemophilic factor VII from human plasma by column chromatographyIndian Journal of Clinical Biochemistry, 18
Irina Knezevic-Maramica, M. Kruskall (2003)
Intravenous immune globulins: an update for cliniciansTransfusion, 43
SS Kuwahara (1980)
Prekallikrein Activator (Hageman Factor Fragment) In Human Plasma FractionsTransfusion, 20
A. Farrugia, P. Poulis (2001)
Intravenous immunoglobulin: regulatory perspectives on use and supplyTransfusion Medicine, 11
Cohn Ej, Strong Le (1946)
Preparation and properties of serum and plasma proteins; a system for the separation into fractions of the protein and lipoprotein components of biological tissues and fluids.Journal of the American Chemical Society, 68
IVIG can be prepared from fractionation of normal human plasma and it is used as a therapeutic drug for treatment of various diseases. IVIG has been for some time the high-growth product within the plasma derived products, at both a global and a national country level. Fractionation was performed according to Cohn method with some modifications. Fraction II was first produced and then it was used for purification and virus inactivation steps. Two methods of virus inactivation (pasteurization at 60°C for 10 hours and solvent/detergent treatment with TnBP and Tween 80) were used and validated. A chromatography method (cation exchange chromatography on CM Sepharose FF) was also added to obtain high purity. The final product (in liquid and freeze dried formulation) meets European Pharmacopeias requirements. The amount of PKA and aggregates was beyond the acceptance limit. The intactness of the IVIG was also examined by circular dichroism (secondary and tertiary structure). It was stable after 6 months of storage. Since Iran market is completely dependant on importation of plasma derived products, it is important to develop such methods for production of IVIG to obtain regional demands.
Human Antibodies – IOS Press
Published: Jan 1, 2010
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