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R. Cote, D. Morrissey, H. Oettgen, L. Old (1984)
Analysis of human monoclonal antibodies derived from lymphocytes of patients with cancer.Federation proceedings, 43 9
K. James, G. Bell (1987)
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J. Boyd, K. James, D. Mcclelland (1984)
Human monoclonal antibodies — production and potentialTrends in Biotechnology, 2
The human monoclonal antibody JDB 1 was examined for reactivity to five breast cancer cell lines as well as normal fibroblasts. The JDB 1 clone secretes both IgG 3 and IgM antibody. In these studies both the total antibody (containing IgG 3 and IgM) as well as the purified IgG 3 and IgM fractions were examined for tumor cell binding reactivity. Peroxidase staining was observed in the breast cancer lines SW527, MCF-7, T47D, SKBR3, and MDAMB231, while GM179 and GM5758 fibroblast lines were negative for antibody binding. Tumor cells were examined using two techniques: a drop cell technique in which cells were fixed onto slides and also a cover slip assay in which cells were grown onto sterile cover slips and subsequently stained with the human monoclonal antibody using a peroxidase technique. Both cytoplasmic and membrane staining were observed for all of the breast tumor cells tested using both assays. In addition, a cellular ELISA was developed and used to quantitate the binding of these antibodies to tumor cells.
Human Antibodies – IOS Press
Published: Jan 1, 1991
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