Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Human monoclonal antibody JDB 1 reacts with cytoplasmic and membrane bound antigens present on a variety of human breast tumor cell lines

Human monoclonal antibody JDB 1 reacts with cytoplasmic and membrane bound antigens present on a... The human monoclonal antibody JDB 1 was examined for reactivity to five breast cancer cell lines as well as normal fibroblasts. The JDB 1 clone secretes both IgG 3 and IgM antibody. In these studies both the total antibody (containing IgG 3 and IgM) as well as the purified IgG 3 and IgM fractions were examined for tumor cell binding reactivity. Peroxidase staining was observed in the breast cancer lines SW527, MCF-7, T47D, SKBR3, and MDAMB231, while GM179 and GM5758 fibroblast lines were negative for antibody binding. Tumor cells were examined using two techniques: a drop cell technique in which cells were fixed onto slides and also a cover slip assay in which cells were grown onto sterile cover slips and subsequently stained with the human monoclonal antibody using a peroxidase technique. Both cytoplasmic and membrane staining were observed for all of the breast tumor cells tested using both assays. In addition, a cellular ELISA was developed and used to quantitate the binding of these antibodies to tumor cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Human Antibodies IOS Press

Human monoclonal antibody JDB 1 reacts with cytoplasmic and membrane bound antigens present on a variety of human breast tumor cell lines

Loading next page...
 
/lp/ios-press/human-monoclonal-antibody-jdb-1-reacts-with-cytoplasmic-and-membrane-5pk0OQvFKU

References (3)

Publisher
IOS Press
Copyright
Copyright © 1991 by IOS Press, Inc
ISSN
1093-2607
eISSN
1875-869X
DOI
10.3233/HAB-1991-2405
Publisher site
See Article on Publisher Site

Abstract

The human monoclonal antibody JDB 1 was examined for reactivity to five breast cancer cell lines as well as normal fibroblasts. The JDB 1 clone secretes both IgG 3 and IgM antibody. In these studies both the total antibody (containing IgG 3 and IgM) as well as the purified IgG 3 and IgM fractions were examined for tumor cell binding reactivity. Peroxidase staining was observed in the breast cancer lines SW527, MCF-7, T47D, SKBR3, and MDAMB231, while GM179 and GM5758 fibroblast lines were negative for antibody binding. Tumor cells were examined using two techniques: a drop cell technique in which cells were fixed onto slides and also a cover slip assay in which cells were grown onto sterile cover slips and subsequently stained with the human monoclonal antibody using a peroxidase technique. Both cytoplasmic and membrane staining were observed for all of the breast tumor cells tested using both assays. In addition, a cellular ELISA was developed and used to quantitate the binding of these antibodies to tumor cells.

Journal

Human AntibodiesIOS Press

Published: Jan 1, 1991

There are no references for this article.