Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Human κ chain expression in a λ phage vector: methods of isolating amplified cDNA affects cloning efficiency

Human κ chain expression in a λ phage vector: methods of isolating amplified cDNA affects cloning... Three common methods of isolating amplified DNA were evaluated for their effects on cloning efficiency in a phage vector designed to express human κ chain. The “glass milk” technique gave higher cloning efficiency and protein expression than phenol-chloroform extraction or microfiltration. This shows that the quality of amplified cDNA should be considered when studying the human antibody repertoire using this vector system. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Human Antibodies IOS Press

Human κ chain expression in a λ phage vector: methods of isolating amplified cDNA affects cloning efficiency

Loading next page...
 
/lp/ios-press/human-chain-expression-in-a-phage-vector-methods-of-isolating-cRTIcfcU9G

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
IOS Press
Copyright
Copyright © 1993 by IOS Press, Inc
ISSN
1093-2607
eISSN
1875-869X
DOI
10.3233/HAB-1993-4302
Publisher site
See Article on Publisher Site

Abstract

Three common methods of isolating amplified DNA were evaluated for their effects on cloning efficiency in a phage vector designed to express human κ chain. The “glass milk” technique gave higher cloning efficiency and protein expression than phenol-chloroform extraction or microfiltration. This shows that the quality of amplified cDNA should be considered when studying the human antibody repertoire using this vector system.

Journal

Human AntibodiesIOS Press

Published: Jan 1, 1993

There are no references for this article.