Human κ chain expression in a λ phage vector: methods of isolating amplified cDNA affects cloning efficiency
Human κ chain expression in a λ phage vector: methods of isolating amplified cDNA affects cloning...
Lazaridis, Konstantinos ; Dang, Howard ; Talal, Norman
1993-01-01 00:00:00
Three common methods of isolating amplified DNA were evaluated for their effects on cloning efficiency in a phage vector designed to express human κ chain. The “glass milk” technique gave higher cloning efficiency and protein expression than phenol-chloroform extraction or microfiltration. This shows that the quality of amplified cDNA should be considered when studying the human antibody repertoire using this vector system.
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngHuman AntibodiesIOS Presshttp://www.deepdyve.com/lp/ios-press/human-chain-expression-in-a-phage-vector-methods-of-isolating-cRTIcfcU9G
Human κ chain expression in a λ phage vector: methods of isolating amplified cDNA affects cloning efficiency
Three common methods of isolating amplified DNA were evaluated for their effects on cloning efficiency in a phage vector designed to express human κ chain. The “glass milk” technique gave higher cloning efficiency and protein expression than phenol-chloroform extraction or microfiltration. This shows that the quality of amplified cDNA should be considered when studying the human antibody repertoire using this vector system.
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