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Gut bacteria are required for the benefits of black raspberries in ApcMin/+ mice

Gut bacteria are required for the benefits of black raspberries in ApcMin/+ mice BACKGROUND:The gut microbiota plays a pivotal role in the development of inflammatory bowel disease and colorectal cancer.OBJECTIVE:To determine whether the gut microbiota is required for the chemoprotective effects of black raspberries (BRBs) in ApcMin/+ mice.METHODS:ApcMin/+ mice were given (a) a control diet for 8 weeks, or (b) the control diet for 4 weeks and then a 5% BRB diet for additional 4 weeks, or (c) the control diet and antibiotics for 4 weeks followed by the 5% BRB diet and antibiotics for the next 4 weeks. At the end of the study, all the mice were euthanized, and colonic and intestinal polyps were counted. mRNA expression levels of TLR4, NF-κ B1, and COX2 were determined in colon and small intestine of these ApcMin/+ mice by quantitative real-time PCR.RESULTS:5% BRBs significantly suppressed intestinal and colonic polyp development in the ApcMin/+ mice, whereas antibiotics significantly abolished BRBs’ chemoprotective effects. BRBs decreased mRNA levels of TLR4, NF-κ B1, and COX2 in colon, whereas significantly enhanced mRNA levels of TLR4 and NF-κ B1 were observed in small intestine of BRB-treated ApcMin/+ mice fed antibiotics.CONCLUSIONS:The gut microbiota is required for BRBs’ chemoprotection against polyp development in ApcMin/+ mice. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Berry Research IOS Press

Gut bacteria are required for the benefits of black raspberries in ApcMin/+ mice

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Publisher
IOS Press
Copyright
Copyright © 2018 © 2018 – IOS Press and the authors. All rights reserved
ISSN
1878-5093
DOI
10.3233/JBR-180337
Publisher site
See Article on Publisher Site

Abstract

BACKGROUND:The gut microbiota plays a pivotal role in the development of inflammatory bowel disease and colorectal cancer.OBJECTIVE:To determine whether the gut microbiota is required for the chemoprotective effects of black raspberries (BRBs) in ApcMin/+ mice.METHODS:ApcMin/+ mice were given (a) a control diet for 8 weeks, or (b) the control diet for 4 weeks and then a 5% BRB diet for additional 4 weeks, or (c) the control diet and antibiotics for 4 weeks followed by the 5% BRB diet and antibiotics for the next 4 weeks. At the end of the study, all the mice were euthanized, and colonic and intestinal polyps were counted. mRNA expression levels of TLR4, NF-κ B1, and COX2 were determined in colon and small intestine of these ApcMin/+ mice by quantitative real-time PCR.RESULTS:5% BRBs significantly suppressed intestinal and colonic polyp development in the ApcMin/+ mice, whereas antibiotics significantly abolished BRBs’ chemoprotective effects. BRBs decreased mRNA levels of TLR4, NF-κ B1, and COX2 in colon, whereas significantly enhanced mRNA levels of TLR4 and NF-κ B1 were observed in small intestine of BRB-treated ApcMin/+ mice fed antibiotics.CONCLUSIONS:The gut microbiota is required for BRBs’ chemoprotection against polyp development in ApcMin/+ mice.

Journal

Journal of Berry Research – IOS Press

Published: Jan 1, 2018

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