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Abstracts of Oral PapersSession 5: Emerging Techniques

Abstracts of Oral PapersSession 5: Emerging Techniques SESSION 5 : EMERGING TECHNIQUES Influence of tissue microenvironments on immune responses in vitro with special reference to immunoglobulin and cytokine production K. James, G. Skibinski and P. Hoffmann* Dept ofSurgery, University of Edinburgh Medical School, Edinburgh, SCOTLAND; and *lnstitutfor lmmunobiologie, Universitiit Freiburg, GERMANY During the past decade, our knowledge of the ceIIular and molecular events associated with key immunological responses has been greatly advanced by the use of isolated sub populations of immunocompetent cells, cloned cell lines, and recombinant derived cytokines Valuable as these studies have been, they do not truly reflect the complex integrative events which take place in both primary and secondary lymphoid tissue both in vivo and in vitro. In order to address this problem, we have developed a tissue explant procedure based on that previously used by others to study T cell maturation in the thymus[I]. This involves culturing uniform slices of lymphoid tissue in a sponge culture system [2J. Using this technique we have observed marked differences in both immunoglobulin and cytokine secretion between explants and suspensions of human spleen. In brief, explants (mitogen stimulated or otherwise) consistently secrete higher levels of immunoglobulin, IL-IB, IL-6, IL-8, and IL-II and exhibit much lower http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Human Antibodies IOS Press

Abstracts of Oral PapersSession 5: Emerging Techniques

Human Antibodies , Volume 7 (2) – Jan 1, 1996
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Publisher
IOS Press
Copyright
Copyright © 1996 by IOS Press, Inc
ISSN
1093-2607
eISSN
1875-869X
DOI
10.3233/HAB-1996-7206
Publisher site
See Article on Publisher Site

Abstract

SESSION 5 : EMERGING TECHNIQUES Influence of tissue microenvironments on immune responses in vitro with special reference to immunoglobulin and cytokine production K. James, G. Skibinski and P. Hoffmann* Dept ofSurgery, University of Edinburgh Medical School, Edinburgh, SCOTLAND; and *lnstitutfor lmmunobiologie, Universitiit Freiburg, GERMANY During the past decade, our knowledge of the ceIIular and molecular events associated with key immunological responses has been greatly advanced by the use of isolated sub populations of immunocompetent cells, cloned cell lines, and recombinant derived cytokines Valuable as these studies have been, they do not truly reflect the complex integrative events which take place in both primary and secondary lymphoid tissue both in vivo and in vitro. In order to address this problem, we have developed a tissue explant procedure based on that previously used by others to study T cell maturation in the thymus[I]. This involves culturing uniform slices of lymphoid tissue in a sponge culture system [2J. Using this technique we have observed marked differences in both immunoglobulin and cytokine secretion between explants and suspensions of human spleen. In brief, explants (mitogen stimulated or otherwise) consistently secrete higher levels of immunoglobulin, IL-IB, IL-6, IL-8, and IL-II and exhibit much lower

Journal

Human Antibodies – IOS Press

Published: Jan 1, 1996

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