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Transport Reversal during Heteroexchange: A Kinetic Study

Transport Reversal during Heteroexchange: A Kinetic Study It is known that secondary transporters, which utilize transmembrane ionic gradients to drive their substrates up a concentration gradient, can reverse the uptake and instead release their substrates. Unfortunately, the Michaelis-Menten kinetic scheme, which is popular in transporter studies, does not include transporter reversal, and it completely neglects the possibility of equilibrium between the substrate concentrations on both sides of the membrane. We have developed a complex two-substrate kinetic model that includes transport reversal. This model allows us to construct analytical formulas allowing the calculation of a “heteroexchange” and “transacceleration” using standard Michaelis coefficients for respective substrates. This approach can help to understand how glial and other cells accumulate substrates without synthesis and are able to release such substrates and gliotransmitters. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biophysics Hindawi Publishing Corporation

Transport Reversal during Heteroexchange: A Kinetic Study

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Publisher
Hindawi Publishing Corporation
Copyright
Copyright © 2013 V. Makarov et al.
ISSN
1687-8000
eISSN
1687-8019
Publisher site
See Article on Publisher Site

Abstract

It is known that secondary transporters, which utilize transmembrane ionic gradients to drive their substrates up a concentration gradient, can reverse the uptake and instead release their substrates. Unfortunately, the Michaelis-Menten kinetic scheme, which is popular in transporter studies, does not include transporter reversal, and it completely neglects the possibility of equilibrium between the substrate concentrations on both sides of the membrane. We have developed a complex two-substrate kinetic model that includes transport reversal. This model allows us to construct analytical formulas allowing the calculation of a “heteroexchange” and “transacceleration” using standard Michaelis coefficients for respective substrates. This approach can help to understand how glial and other cells accumulate substrates without synthesis and are able to release such substrates and gliotransmitters.

Journal

Journal of BiophysicsHindawi Publishing Corporation

Published: Oct 26, 2013

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