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Fluorometer Controlled Apparatus Designed for Long-Duration Algal-Feeding Experiments and Environmental Effect Studies with Mussels

Fluorometer Controlled Apparatus Designed for Long-Duration Algal-Feeding Experiments and... Experimental feeding and growth studies on filter-feeding organisms often rely on constant algal concentrations maintained over extended periods of time. Here we present a fluorometer controlled apparatus (FCA) designed for feeding experiments with suspension-feeding mussels at naturally low chlorophyll concentrations above 0.5 µg L−1. The principle used is feedback regulation of the algal concentration based on continuous monitoring of the fluorescence intensity of chlorophyll in water pumped through the apparatus from an aquarium with mussels. The filtration rate is monitored continuously as the rate of change of measured volume of an algal stock added to the aquarium for keeping a constant algal concentration. As an example, the FCA has been used to study the filtration rates of blue mussels (Mytilus edulis) at algal concentrations both near and above the incipient saturation level for reduced filtration activity. As another example to put the FCA into perspective as a reliable method for environmental effect studies, the apparatus has been used to demonstrate the acute effect of changing salinity on the filtration rate of M. edulis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Marine Biology Hindawi Publishing Corporation

Fluorometer Controlled Apparatus Designed for Long-Duration Algal-Feeding Experiments and Environmental Effect Studies with Mussels

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Publisher
Hindawi Publishing Corporation
Copyright
Copyright © 2013 Daniel Pleissner et al.
ISSN
1687-9481
eISSN
1687-949X
Publisher site
See Article on Publisher Site

Abstract

Experimental feeding and growth studies on filter-feeding organisms often rely on constant algal concentrations maintained over extended periods of time. Here we present a fluorometer controlled apparatus (FCA) designed for feeding experiments with suspension-feeding mussels at naturally low chlorophyll concentrations above 0.5 µg L−1. The principle used is feedback regulation of the algal concentration based on continuous monitoring of the fluorescence intensity of chlorophyll in water pumped through the apparatus from an aquarium with mussels. The filtration rate is monitored continuously as the rate of change of measured volume of an algal stock added to the aquarium for keeping a constant algal concentration. As an example, the FCA has been used to study the filtration rates of blue mussels (Mytilus edulis) at algal concentrations both near and above the incipient saturation level for reduced filtration activity. As another example to put the FCA into perspective as a reliable method for environmental effect studies, the apparatus has been used to demonstrate the acute effect of changing salinity on the filtration rate of M. edulis.

Journal

Journal of Marine BiologyHindawi Publishing Corporation

Published: Nov 6, 2013

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