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The influence of doxazosin on the contractility of the urinary bladder in female pigs with experimentally induced cystitis

The influence of doxazosin on the contractility of the urinary bladder in female pigs with... AbstractThe present in vitro study investigated the influence of doxazosin on the contractility of the urinary bladder in female pigs with experimentally induced cystitis. Fifteen juvenile female piglets (18-20 kg body weight) were randomly assigned into three groups (n=5 animals each): i) control (clinically healthy animals, without doxazosin treatment), ii) animals with induced inflammation of the urinary bladder, but without doxazosin treatment (experimental group I) and iii) animals with inflamed bladder, treated orally with doxazosin (0.1 mg/kg body weight for 30 days; experimental group II). Thereafter, the pigs were sacrificed and strips of the bladder trigone were suspended in organ baths. The tension and amplitude of the smooth muscles was measured before and after exposition to 5-hydroxytryptamine (5-HT; 10-6-10-4 M), acetylocholine (ACh; 10-5-10-3 M) and norepinephrine (NE; 10-9-10-7 M). 5-HT caused an increase in the tension of contractions in all the groups and the amplitude in the experimental groups, however, the effect was higher in the experimental group I than in group II as compared to that found in the pre-treatment period. ACh caused an increase in the tension in the control group and a decrease in the amplitude in both experimental groups; these changes significantly differed between the control and doxazosin-treated group. NE caused a decrease in the tension in both experimental groups and amplitude in all the groups, however, the effect was most strongly expressed in doxazosine-treated group. The present study has revealed that long-term administration of doxazosin causes a desensitization of the detrusor smooth muscle to in vitro applied mediators in the autonomic nervous system. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Polish Journal of Veterinary Science de Gruyter

The influence of doxazosin on the contractility of the urinary bladder in female pigs with experimentally induced cystitis

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Publisher
de Gruyter
Copyright
© Polish Academy of Sciences, Committee of Veterinary Sciences &University of Warmia and Mazury in Olsztyn
ISSN
2300-2557
eISSN
2300-2557
DOI
10.1515/pjvs-2017-0058
Publisher site
See Article on Publisher Site

Abstract

AbstractThe present in vitro study investigated the influence of doxazosin on the contractility of the urinary bladder in female pigs with experimentally induced cystitis. Fifteen juvenile female piglets (18-20 kg body weight) were randomly assigned into three groups (n=5 animals each): i) control (clinically healthy animals, without doxazosin treatment), ii) animals with induced inflammation of the urinary bladder, but without doxazosin treatment (experimental group I) and iii) animals with inflamed bladder, treated orally with doxazosin (0.1 mg/kg body weight for 30 days; experimental group II). Thereafter, the pigs were sacrificed and strips of the bladder trigone were suspended in organ baths. The tension and amplitude of the smooth muscles was measured before and after exposition to 5-hydroxytryptamine (5-HT; 10-6-10-4 M), acetylocholine (ACh; 10-5-10-3 M) and norepinephrine (NE; 10-9-10-7 M). 5-HT caused an increase in the tension of contractions in all the groups and the amplitude in the experimental groups, however, the effect was higher in the experimental group I than in group II as compared to that found in the pre-treatment period. ACh caused an increase in the tension in the control group and a decrease in the amplitude in both experimental groups; these changes significantly differed between the control and doxazosin-treated group. NE caused a decrease in the tension in both experimental groups and amplitude in all the groups, however, the effect was most strongly expressed in doxazosine-treated group. The present study has revealed that long-term administration of doxazosin causes a desensitization of the detrusor smooth muscle to in vitro applied mediators in the autonomic nervous system.

Journal

Polish Journal of Veterinary Science – de Gruyter

Published: Sep 26, 2017

References

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    Khan
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    Yoshimura
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    Callahan
  • How widespread are the symptoms of an overactive bladder and how are they managed population - based prevalence study
    Milsom
  • Pharmacological analysis of hydroxytryptamine effects on electrically stimulated human isolated urinary bladder
    Corsi
  • The standardisation of terminology of lower urinary tract function : report from the standardisation sub - committee of the International Continence
    Abrams
  • Urinary bladder contraction relaxation pathophysiology
    Andersson
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    Nakamura
  • cell involvement in interstitial cystitis a review of human and experimental evidence
    Theoharides
  • Characterization of prejunctional serotonin receptors modulating acetylcholine release in the human detrusor
    Agostino
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    Liu
  • The standardisation of terminology of lower urinary tract function : report from the standardisation sub - committee of the International Continence
    Abrams
  • DA of NGF in mouse urothelium leads to neuronal hyperinnervation pelvic sensitivity and changes in urinary bladder function
    Schnegelsberg
  • Neural upregulation in interstitial cystitis
    Nazif
  • The influence of doxazosin an alpha adrenergic receptor antagonist on the urinary bladder contractility in pigs
    Markiewicz
  • Membrane properties of the smooth muscle membrane of the guinea - pig urinary bladder
    Creed
  • Increased severity of inflammation correlates with elevated expression of nerve fibers and nerve growth factor on interstitial cystitis / bladder pain syndrome
    Liu
  • cell involvement in interstitial cystitis a review of human and experimental evidence
    Theoharides
  • Influence of serotonin on lower urinary tract smooth muscle in vitro
    Klarskov
  • Swine as models in biomedical research and toxicology testing
    Swindle
  • Electrical and mechanical activity recorded from rabbit urinary bladder in response to nerve stimulation
    Creed
  • The role of central hydroxytryptamine receptors in the control of micturition
    Ramage
  • WC Neural control of the lower urinary tract
    De Groat
  • and activity augment brain - derived neurotrophic factor peripheral release
    Qiao
  • Influence of serotonin on lower urinary tract smooth muscle in vitro
    Klarskov
  • and activity augment brain - derived neurotrophic factor peripheral release
    Qiao
  • Neural receptors in the human isolated detrusor muscle : effects of indole benzimidazolone and substituted benzamide agonists and antagonists
    Candura
  • Alpha alpha adrenoceptors mediate opposing actions on parasympathetic neurons
    Nakamura
  • Urinary bladder contraction relaxation pathophysiology
    Andersson
  • Urodynamics in acetone - induced cystitis of anesthetized rats
    Shimizu
  • The influence of doxazosin an alpha adrenergic receptor antagonist on the urinary bladder contractility in pigs
    Markiewicz
  • Electrical and mechanical activity of the isolated lower urinary tract of the guinea - pig
    Callahan
  • Differential release of serotonin and histamine from mast cells
    Theoharides
  • How widespread are the symptoms of an overactive bladder and how are they managed population - based prevalence study
    Milsom
  • Characterization of prejunctional serotonin receptors modulating acetylcholine release in the human detrusor
    Agostino
  • WC Neural control of the lower urinary tract
    De Groat
  • CH of the lower urinary tract
    Hennenberg
  • GT de WC Prejunctional facilitatory alpha adrenoceptors in the rat urinary bladder
    Somogyi
  • Neural upregulation in interstitial cystitis
    Nazif
  • Electrical and mechanical activity recorded from rabbit urinary bladder in response to nerve stimulation
    Creed
  • Hydroxytryptamine - induced potentiation of cholinergic responses to electrical field stimulation in pig detrusor muscle
    Sellers
  • de WC Sympathetic modulation of cholinergic transmission in cat vesical ganglia is mediated by alpha alpha adrenoceptors
    Keast
  • Mediation of micturition reflex by central norepinephrine from the locus coeruleus in the cat
    Yoshimura
  • Swine as models in biomedical research and toxicology testing
    Swindle
  • Urodynamics in acetone - induced cystitis of anesthetized rats
    Shimizu
  • Neural receptors in the human isolated detrusor muscle : effects of indole benzimidazolone and substituted benzamide agonists and antagonists
    Candura
  • DA of NGF in mouse urothelium leads to neuronal hyperinnervation pelvic sensitivity and changes in urinary bladder function
    Schnegelsberg
  • Hydroxytryptamine - induced potentiation of cholinergic responses to electrical field stimulation in pig detrusor muscle
    Sellers