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The effect of cold shock on the immune response of the greater wax moth Galleria mellonella after infection with entomopathogenic bacteria Bacillus thuringiensis / Wpływ szoku zimna na odpowiedź immunologiczną barciaka większego Galleria mellonella po zakażeniu bakterią entomopatogenną Bacillus thuringiensis

The effect of cold shock on the immune response of the greater wax moth Galleria mellonella after... Abstract Insect immune system consists of only innate mechanisms relied on cellular and humoral branches. Many defence proteins and peptides exist or appear in response to infection in insect’s hemolymph. The interaction between the infected host and the entomopathogen occurs in the conditions of external environment. In this work the greater wax moth larvae of Galleria mellonella were subjected to a temperature of 120C for a short period of time, directly before infection with entomopathogenic bacteria Bacillus thuringiensis. It appeared that the induction of the immune response was higher in cold-shocked animals than in larvae permanently reared at the optimal temperature of 28 0C. This enhanced immune response was manifested as higher antibacterial and lysozyme-type activity detected in full hemolymph, and as a higher level of peptides of molecular weight below 10 kDa having antibacterial activity. Moreover, other changes in the contents of proteins in the hemolymph were observed. These changes concerned inter alia apolipophorin III, the multifunctional protein of immune significance. Its level was higher in the hemolymph of animals pre-exposed to cold shock than in nonshocked, infected ones. Altogether our results indicate that the interdependence mechanisms occur between cold shock and the immune response. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annales UMCS, Biologia de Gruyter

The effect of cold shock on the immune response of the greater wax moth Galleria mellonella after infection with entomopathogenic bacteria Bacillus thuringiensis / Wpływ szoku zimna na odpowiedź immunologiczną barciaka większego Galleria mellonella po zakażeniu bakterią entomopatogenną Bacillus thuringiensis

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Publisher
de Gruyter
Copyright
Copyright © 2015 by the
ISSN
2083-3563
eISSN
2083-3563
DOI
10.1515/umcsbio-2015-0001
Publisher site
See Article on Publisher Site

Abstract

Abstract Insect immune system consists of only innate mechanisms relied on cellular and humoral branches. Many defence proteins and peptides exist or appear in response to infection in insect’s hemolymph. The interaction between the infected host and the entomopathogen occurs in the conditions of external environment. In this work the greater wax moth larvae of Galleria mellonella were subjected to a temperature of 120C for a short period of time, directly before infection with entomopathogenic bacteria Bacillus thuringiensis. It appeared that the induction of the immune response was higher in cold-shocked animals than in larvae permanently reared at the optimal temperature of 28 0C. This enhanced immune response was manifested as higher antibacterial and lysozyme-type activity detected in full hemolymph, and as a higher level of peptides of molecular weight below 10 kDa having antibacterial activity. Moreover, other changes in the contents of proteins in the hemolymph were observed. These changes concerned inter alia apolipophorin III, the multifunctional protein of immune significance. Its level was higher in the hemolymph of animals pre-exposed to cold shock than in nonshocked, infected ones. Altogether our results indicate that the interdependence mechanisms occur between cold shock and the immune response.

Journal

Annales UMCS, Biologia – de Gruyter

Published: Mar 1, 2015

References

  • Is innate enough The innate immune response in Drosophila Comp Trend
    Irving
  • Cleavage of structural proteins during the assembly of the head of bacteriophage
    Laemmli
  • kappa in the immune response of Drosophila Cold a
    Hetru
  • Mode of action and specificity of Bacillus thuringiensis toxins in the control of caterpillars and stink bugs in soybean culture
    Schünemann
  • Lower temperature influences developmental rhythms of the wax moth Galleria mellonella Putative role of ecdysteroids
    Mikołajczyk
  • Purification and characterization of eight peptides from Galleria mellonella immune hemolymph
    Cytrynska
  • Immunoreaktionen bei Insekten Lysozym als grundlegender antibakterieller Faktor im humoralen Abwehrmechanismus der Insekten Biologische Zentralblatt
    Mohrig
  • Desiccation elicits heat shock protein transcription in the Xesh crassipalpis but does not enhance tolerance to high or low temperatures
    Tammariello
  • mellonella as a model host to study Paracoccidioides lutzii and Histoplasma capsulatum Virulence
    Thomaz
  • insect immune response
    Zdybicka
  • is a pleiotropic regulator of extracellular virulence factor gene expression in Bacillus thuringiensis
    Agaisse
  • Overview of Drosophila immunity a historical perspective
    Imler
  • Effects of two hemolymph proteins on humoral defense reactions in the wax moth mellonella Immun
    Park
  • eds Bacillus thuringiensis An Environmental Theory Practice
    Entwistle
  • Mode of action and specificity of Bacillus thuringiensis toxins in the control of caterpillars and stink bugs in soybean culture
    Schünemann
  • Influence of low temperature on the synthesis of some Galleria mellonella proteins
    Kłudkiewicz
  • emani Mechanisms of Drosophila Dscam mutually exclusive splicing regulation
    Soller
  • Immunoreaktionen bei Insekten Lysozym als grundlegender antibakterieller Faktor im humoralen Abwehrmechanismus der Insekten Biologische Zentralblatt
    Mohrig
  • Involvement of apolipophorin III in antibacterial defense of Galleria mellonella larvae
    Zdybicka
  • eds Bacillus thuringiensis An Environmental Theory Practice
    Entwistle
  • emani Mechanisms of Drosophila Dscam mutually exclusive splicing regulation
    Soller
  • Bacillus thuringiensis story of a successful bioinsecticide
    Bravo
  • Regulators and signalling in insect haemocyte immunity
    Marmaras
  • mellonella as a model host for human pathogens : recent studies and new perspectives Virulence
    Junqueira
  • During The non - enzymatic microbicidal activity of lysozymes
    Porsch
  • Bacillus thuringiensis story of a successful bioinsecticide
    Bravo
  • Humoral immune response of Galleria mellonella larvae after infection by Beauveria bassiana under optimal and heat - shock conditions
    Wojda
  • Changes in the hemolymph protein profiles in Galleria mellonella infected with Bacillus thuringiensis involve apolipophorin III The effect of heat - shock Arch - in press
    Taszłow
  • comprehensive transcriptome and immune - gene repertoire of the lepidopteran model host Galleria mellonella
    Vogel
  • von - sodium dodecyl sulfate - polyacrylamide gel electrophoresis for the separation of proteins in the range from to kDa
    Schagger
  • The insect Galleria mellonella as a powerful infection model to investigate bacterial pathogenesis of Visualized Experiments
    Ramarao
  • Quantification of antimicrobial activity using the inhibition - zone assay In editors Techniques in Fair Heaven : SOS Publications
    Hultmark