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Biogenesis and interconversion of substituted tetrahydrofolates
C. Lovell, A. Przybyla, L. Ljungdahl (1990)
Primary structure of the thermostable formyltetrahydrofolate synthetase from Clostridium thermoaceticum.Biochemistry, 29 24
(1986)
Nucleotide sequence of the Saccharornyces cerevisiae ADE 3 gene encoding C \ - tetrahydrofolate synthase
C. Kirk, H. Imeson, Li-li Zheng, E. Cossins (1994)
The affinity of pea cotyledon 10-formyltetrahydrofolate synthetase for polyglutamate substratesPhytochemistry, 35
W. Campbell, G. Gowri (1990)
Codon usage in higher plants, green algae, and cyanobacteria.Plant physiology, 92 1
(1992)
Isolation and sequencing of the cON A coding for spinach 10 - formyltetrahydrofOiate synthetase
D. Hum, A. Bell, R. Rozen, R. Mackenzie (1988)
Primary structure of a human trifunctional enzyme. Isolation of a cDNA encoding methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase.The Journal of biological chemistry, 263 31
N. Mejia, R. Mackenzie (1988)
NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase in transformed cells is a mitochondrial enzyme.Biochemical and biophysical research communications, 155 1
C. Barlowe, D. Appling (1990)
Molecular genetic analysis of Saccharomyces cerevisiae C1-tetrahydrofolate synthase mutants reveals a noncatalytic function of the ADE3 gene product and an additional folate-dependent enzymeMolecular and Cellular Biology, 10
B. Vogelstein, Delbert Gillespie (1979)
Preparative and analytical purification of DNA from agarose.Proceedings of the National Academy of Sciences of the United States of America, 76 2
D. Appling (1991)
Compartmentation of folate‐mediated one‐carbon metabolism in eukaryotesThe FASEB Journal, 5
C. Kirk, Liangfu Chen, H. Imeson, E. Cossins (1995)
A 5,10-methylenetetrahydrofolate dehydrogenase: 5,10-methenyltetrahydrofolate cyclohydrolase protein from Pisum sativumPhytochemistry, 39
L. Chen, S. Chan, E. Cossins (1997)
Distribution of Folate Derivatives and Enzymes for Synthesis of 10-Formyltetrahydrofolate in Cytosolic and Mitochondrial Fractions of Pea Leaves, 115
J. Nour, J. Rabinowitz (1991)
Isolation, characterization, and structural organization of 10-formyltetrahydrofolate synthetase from spinach leaves.The Journal of biological chemistry, 266 27
A. Thigpen, M. West, D. Appling (1990)
Rat C1-tetrahydrofolate synthase. cDNA isolation, tissue-specific levels of the mRNA, and expression of the protein in yeast.The Journal of biological chemistry, 265 14
(1993)
Cloning and characteriz , uion of the Saccharomyces cerevisiae gene l ' I 1 wding ~ AD - dependent 5 , 1 O - methylene - tetrahydro - t ( ) btl ' ddwdrogenase
C. Joshi (1987)
An inspection of the domain between putative TATA box and translation start site in 79 plant genes.Nucleic acids research, 15 16
P. Pawelek, R. Mackenzie (1996)
Methylenetetrahydrofolate dehydrogenase-cyclohydrolase from Photobacterium phosphoreum shares properties with a mammalian mitochondrial homologue.Biochimica et biophysica acta, 1296 1
(1991)
Will < inson M . Purification of RNA
H. Imeson, Li-li Zheng, E. Cossins (1990)
Folylpolyglutamate Derivatives of Pisum sativum L. Determination of Polyglutamate Chain Lengths by High Performance Liquid Chromatography Following Conversion to p-aminobenzoylpolyglutamatesPlant and Cell Physiology, 31
C. Belangér, R MacKenzie (1989)
Isolation and characterization of cDNA clones encoding the murine NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase.The Journal of biological chemistry, 264 9
C. Wagner (1996)
Symposium on the subcellular compartmentation of folate metabolism.The Journal of nutrition, 126 4 Suppl
L. D'Ari, J. Rabinowitz (1991)
Purification, characterization, cloning, and amino acid sequence of the bifunctional enzyme 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase from Escherichia coli.The Journal of biological chemistry, 266 35
D. Appling, Jesse Rabinowitz (1985)
Evidence for overlapping active sites in a multifunctional enzyme: immunochemical and chemical modification studies on C1-tetrahydrofolate synthase from Saccharomyces cerevisiae.Biochemistry, 24 14
D. Drummond, S. Smith, R. Mackenzie (1983)
Methylenetetrahydrofolate dehydrogenase - methenyltetrahydrofolate cyclohydrolase - formyltetrahydrofolate synthetase from porcine liver: evidence to support a common dehydrogenase-cyclohydrolase site.Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire, 61 11
T. Whitehead, J. Rabinowitz (1988)
Nucleotide sequence of the Clostridium acidiurici ("Clostridium acidi-urici") gene for 10-formyltetrahydrofolate synthetase shows extensive amino acid homology with the trifunctional enzyme C1-tetrahydrofolate synthase from Saccharomyces cerevisiaeJournal of Bacteriology, 170
K. Shannon, J. Rabinowitz (1986)
Purification and characterization of a mitochondrial isozyme of C1-tetrahydrofolate synthase from Saccharomyces cerevisiae.The Journal of biological chemistry, 261 26
E. Cossins, Liangfu Chen (1997)
Folates and one-carbon metabolism in plants and fungi.Phytochemistry, 45 3
Summary In plant cells, the interconversion of formyl- and methylene-tetrahydrofolates is catalyzed by a bifunctional protein possessing methenyltetrallydrofolate cydohydrolase (EC 3.5.4.9) and methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities. The present work reports the isolation and sequencing of a cDNA that encodes this protein. Polydonal antibodies, raised against purified pea cytosolic dehydrogenase:cyclohydrolase, were used to screen a λgt 11 cDNA expression library, constructed from leaf extracts of this species. The screen identified a phage containing a cDNA insert with an open reading frame encoding a 294 amino acid protein (M r 31,344). The deduced primary structure of this protein contained most of the conserved regions found in other dehydrogenase:cyclohydrolase proteins including the corresponding domains of the trifunctional C 1 -THF synthases of mammalian and yeast origins.
Pteridines – de Gruyter
Published: Nov 1, 1999
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