Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

In Vitro Regeneration Potential of Seven Commercial Soybean Cultivars (Glycine max L.) for Use in Biotechnology

In Vitro Regeneration Potential of Seven Commercial Soybean Cultivars (Glycine max L.) for Use in... Abstract This work is aimed to evaluate in vitro regeneration potential of seven commercial soybean varieties Bohemians, Cardiff, Gallec, Merlin, Moravians, Naya and Silensia (Glycine max L.) cultivated in Central Europe. Our results showed the half-seeds could be effectively used as an explant source for all tested cultivars. The regeneration was initiated on the media containing growth regulators 1.67 mg.l-1 BAP and 0.25 mg.l -1 GA 3 . Within the first five days culture, green chlorophyll-containing explants were observed with frequency from 18.3% to 55.9%. Two weeks later, the explants responded by production of calli with the efficiency up to 83.0%. First shoots appeared after 2–3 weeks of subculture on the media. The soybean regeneration showed to be genotype-dependent with variable efficiencies from 5.7% (cv. Naya) to 37.7% (cv. Gallec). The cultivars Cardiff, Merlin and Gallec appear to be the most promising candidates for further biotechnological use. Application of antioxidants such as L-cysteine, dithiothreitol and sodium thiosulfate does not have effect on the explant regeneration for the first five days. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nova Biotechnologica et Chimica de Gruyter

In Vitro Regeneration Potential of Seven Commercial Soybean Cultivars (Glycine max L.) for Use in Biotechnology

Loading next page...
 
/lp/de-gruyter/in-vitro-regeneration-potential-of-seven-commercial-soybean-cultivars-Bc3sw6mx0q
Publisher
de Gruyter
Copyright
Copyright © 2016 by the
ISSN
1338-6905
eISSN
1338-6905
DOI
10.1515/nbec-2016-0001
Publisher site
See Article on Publisher Site

Abstract

Abstract This work is aimed to evaluate in vitro regeneration potential of seven commercial soybean varieties Bohemians, Cardiff, Gallec, Merlin, Moravians, Naya and Silensia (Glycine max L.) cultivated in Central Europe. Our results showed the half-seeds could be effectively used as an explant source for all tested cultivars. The regeneration was initiated on the media containing growth regulators 1.67 mg.l-1 BAP and 0.25 mg.l -1 GA 3 . Within the first five days culture, green chlorophyll-containing explants were observed with frequency from 18.3% to 55.9%. Two weeks later, the explants responded by production of calli with the efficiency up to 83.0%. First shoots appeared after 2–3 weeks of subculture on the media. The soybean regeneration showed to be genotype-dependent with variable efficiencies from 5.7% (cv. Naya) to 37.7% (cv. Gallec). The cultivars Cardiff, Merlin and Gallec appear to be the most promising candidates for further biotechnological use. Application of antioxidants such as L-cysteine, dithiothreitol and sodium thiosulfate does not have effect on the explant regeneration for the first five days.

Journal

Nova Biotechnologica et Chimicade Gruyter

Published: Jun 1, 2016

References