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In-vitro efficacy of indocyanine green-mediated photodynamic therapy in combination with cisplatin or etoposide

In-vitro efficacy of indocyanine green-mediated photodynamic therapy in combination with... Abstract: Localizing the cytotoxic effects of cancer therapies to only affect the tumor cells is a goal in oncology, to maximize efficacy and minimize treatment-related morbidities. Most effective chemotherapeutic drugs have significant side effects due to off-target toxicity. By comparison, photodynamic therapy (PDT) is a localized therapy without significant systemic toxicity but may have limited efficacy. Hence, combining PDT with chemotherapy was investigated to determine if the anti-tumor effect of the latter could be enhanced. PDT using indocyanine green (ICG), activated by near-infrared light, was investigated in lung tumor cells in vitro in combination with cisplatin or etoposide (VP-16). The combination of cisplatin and ICG-PDT had significant concentration-dependent dark toxicity, with little additional cell kill after light exposure. Conversely, combination therapy comprising 5 μ m VP-16, 50 μ m ICG and 50 J/cm 2 808-nm radiant exposure resulted in ~10% clonogenic cell survival compared to ~80% cell survival with either treatment alone. This potentially synergistic gain was achieved only when both treatments were given at the same time or when VP-16 was administered 4 h prior to PDT. VP-16 given 4 h post PDT did not show any added benefit over PDT alone. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Photonics & Lasers in Medicine de Gruyter

In-vitro efficacy of indocyanine green-mediated photodynamic therapy in combination with cisplatin or etoposide

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References (59)

Publisher
de Gruyter
Copyright
Copyright © 2015 by the
ISSN
2193-0635
eISSN
2193-0643
DOI
10.1515/plm-2015-0015
Publisher site
See Article on Publisher Site

Abstract

Abstract: Localizing the cytotoxic effects of cancer therapies to only affect the tumor cells is a goal in oncology, to maximize efficacy and minimize treatment-related morbidities. Most effective chemotherapeutic drugs have significant side effects due to off-target toxicity. By comparison, photodynamic therapy (PDT) is a localized therapy without significant systemic toxicity but may have limited efficacy. Hence, combining PDT with chemotherapy was investigated to determine if the anti-tumor effect of the latter could be enhanced. PDT using indocyanine green (ICG), activated by near-infrared light, was investigated in lung tumor cells in vitro in combination with cisplatin or etoposide (VP-16). The combination of cisplatin and ICG-PDT had significant concentration-dependent dark toxicity, with little additional cell kill after light exposure. Conversely, combination therapy comprising 5 μ m VP-16, 50 μ m ICG and 50 J/cm 2 808-nm radiant exposure resulted in ~10% clonogenic cell survival compared to ~80% cell survival with either treatment alone. This potentially synergistic gain was achieved only when both treatments were given at the same time or when VP-16 was administered 4 h prior to PDT. VP-16 given 4 h post PDT did not show any added benefit over PDT alone.

Journal

Photonics & Lasers in Medicinede Gruyter

Published: Nov 1, 2015

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