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Glyceryl-ether Monooxygenase (EC 1.14.16.5J Part V: Some Aspects of the Stoichiometry

Glyceryl-ether Monooxygenase (EC 1.14.16.5J Part V: Some Aspects of the Stoichiometry Summary A stoichiometry of one is found for the reaction between RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin catalysed by glyceryl-ether monooxygenase during the first two minutes. The apparent decoupling of these two substrates after the first two minutes of reaction is discussed. The stoichiometry of the oxidation of 6-methyl-5,6,7,8-tetrahydropterin (to quinonoid 6-methyl-7,8(6H)-dihydropterinJ to the conversion of NADH to NAD in the monooxygenase-dihydropteridine reductase coupled reaction is ca one at concentrations of 6-methyl-5,6,7,8-tetrahydropterin less than 100 μM. At higher concentrations of the tetrahydropterin the ratio decreases and it is shown to be due to product inhibition of the reductase. R( +)- and S( - )-6-Methyl- 5,6,7,8-tetrahydropterin are about equally effective substrates for glyceryl-ether monooxygenase. A new and convenient synthesis of 1- 14 C-batyl alcohol is reported. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Pteridines de Gruyter

Glyceryl-ether Monooxygenase (EC 1.14.16.5J Part V: Some Aspects of the Stoichiometry

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Publisher
de Gruyter
Copyright
Copyright © 1994 by the
ISSN
0933-4807
eISSN
2195-4720
DOI
10.1515/pteridines.1994.5.1.1
Publisher site
See Article on Publisher Site

Abstract

Summary A stoichiometry of one is found for the reaction between RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin catalysed by glyceryl-ether monooxygenase during the first two minutes. The apparent decoupling of these two substrates after the first two minutes of reaction is discussed. The stoichiometry of the oxidation of 6-methyl-5,6,7,8-tetrahydropterin (to quinonoid 6-methyl-7,8(6H)-dihydropterinJ to the conversion of NADH to NAD in the monooxygenase-dihydropteridine reductase coupled reaction is ca one at concentrations of 6-methyl-5,6,7,8-tetrahydropterin less than 100 μM. At higher concentrations of the tetrahydropterin the ratio decreases and it is shown to be due to product inhibition of the reductase. R( +)- and S( - )-6-Methyl- 5,6,7,8-tetrahydropterin are about equally effective substrates for glyceryl-ether monooxygenase. A new and convenient synthesis of 1- 14 C-batyl alcohol is reported.

Journal

Pteridinesde Gruyter

Published: Feb 1, 1994

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