Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Determination of methylxanthines in tea samples by HPLC method

Determination of methylxanthines in tea samples by HPLC method AbstractMethylxanthines such as caffeine, theophylline, theobromine are significant and widespread psychoactive substances. We developed the isocratic method with optimum composition of the mobile phase 90 % water: 10 % acetonitrile and confirmed repeatability of retention times and peak areas. The developed HPLC method was applied to determine the content of methylxanthines in selected types of black and green teas available on the market. Of the black teas (tea bags), the highest concentration of theobromine was found in Ceylon tea (18.98 mg.L−1). The highest concentration of caffeine was in a cup of Earl Gray tea (254.09 mg.L−1). Among loose black teas, the highest content of both theobromine and caffeine was found in Pu Erh Superior tea, where the theobromine content was 24.62 mg.L−1 and the caffeine content was 520.67 mg.L−1. Of green powder teas, highest caffeine content (306.46 mg.L−1) was in Shizuoka Matcha Premium and the highest content of theobromine (8.45 mg.L−1) was found in GABA Midori. From the loose green tea, the highest concentration of theobromine (12.85 mg.L−1) was in Lung Ching West Lake. The highest caffeine content (484.85 mg.L−1) was in Gyokuro Shizuoka Premium Tea. In both types of teas the amount of theobromine and caffeine was quantified, but the presence of theophylline was not proven. Data on contents of these metabolites in tea products are highly informative for consumers. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nova Biotechnologica et Chimica de Gruyter

Determination of methylxanthines in tea samples by HPLC method

Download PDF
9 pages

Loading next page...
 
/lp/de-gruyter/determination-of-methylxanthines-in-tea-samples-by-hplc-method-uH5qDL7kpa
Publisher
de Gruyter
Copyright
© 2019 Timotej Jankech et al., published by Sciendo
ISSN
1338-6905
eISSN
1339-004X
DOI
10.2478/nbec-2019-0015
Publisher site
See Article on Publisher Site

Abstract

AbstractMethylxanthines such as caffeine, theophylline, theobromine are significant and widespread psychoactive substances. We developed the isocratic method with optimum composition of the mobile phase 90 % water: 10 % acetonitrile and confirmed repeatability of retention times and peak areas. The developed HPLC method was applied to determine the content of methylxanthines in selected types of black and green teas available on the market. Of the black teas (tea bags), the highest concentration of theobromine was found in Ceylon tea (18.98 mg.L−1). The highest concentration of caffeine was in a cup of Earl Gray tea (254.09 mg.L−1). Among loose black teas, the highest content of both theobromine and caffeine was found in Pu Erh Superior tea, where the theobromine content was 24.62 mg.L−1 and the caffeine content was 520.67 mg.L−1. Of green powder teas, highest caffeine content (306.46 mg.L−1) was in Shizuoka Matcha Premium and the highest content of theobromine (8.45 mg.L−1) was found in GABA Midori. From the loose green tea, the highest concentration of theobromine (12.85 mg.L−1) was in Lung Ching West Lake. The highest caffeine content (484.85 mg.L−1) was in Gyokuro Shizuoka Premium Tea. In both types of teas the amount of theobromine and caffeine was quantified, but the presence of theophylline was not proven. Data on contents of these metabolites in tea products are highly informative for consumers.

Journal

Nova Biotechnologica et Chimica – de Gruyter

Published: Dec 1, 2019

References

  • de EG mares MV Impact of caffeine and coffee on our Trends
    Mejia
  • Simultaneous determination of phenolic compounds and caffeine in teas and mate using RP UV detection : Method development and optimization of extraction process
    Bae
  • HPLC method for urinary theobromine determination : Effect of consumption of cocoa products on theobromine urinary excretion in children
    Rodriguez
  • Simultaneous determination of caffeine theophylline and theobromine in food samples by a kinetic spectrophotometric method
    Kokot
  • Quantitative determination of caffeine in different matrices Mac
    Grujić
  • new method based on electrospray ionisation ion mobility spectrometry for simultaneous determination of caffeine and theophylline
    Jafari
  • Rapid determination of methylxanthines in real samples by high - performance liquid chromatography using the new FastGradient narrow - bore monolithic column
    Tzanavaras
  • CM of caffeine xanthine and the dimethyl xanthines
    Gibson
  • Simultaneous HPLC determination of caffeine theobromine and theophylline in food drinks and herbal products
    Srdjenovic
  • Estimation of caffeine in different brands of energy drinks by Ultra - Violet spectroscopy
    Mufakkar