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Carbohydrate-protein interactions characterized by dual polarization hybrid plasmonic waveguide

Carbohydrate-protein interactions characterized by dual polarization hybrid plasmonic waveguide AbstractOptical biosensors present good performance for the affinity analysis of a molecular binding event. However, most of those excited with single optical mode are “blind” to the conformational change of bound molecules. We theoretically demonstrate a dual polarization hybrid plasmonic (DPHP) waveguide with nano-slots. By addressing the structure with dual polarizations, the optogeometrical properties (density and thickness) of protein layers have been determined without ambiguity. Differences in the hybrid mode dispersion between the transverse electric (TE) and transverse magnetic (TM) modes allow to separately determine the thickness and the density at all stages during the molecular interaction. In addition, nano-slots can be equated with an effective optical capacitance resulting in a strong field confinement; thereby, subtle changes in the ambient medium can be sensed. A proof of concept is conducted by analyzing the conformational change of HepV, a recombinant fragment of collagen V, during complicated molecular interaction. Integrated with adlayer thickness and density, we can conclude that a thick sparse layer formed after heparin capture and a thin dense layer arising from HepV bound. Accordingly, HepV undergoing conformational change has been traced and verified as molecular interaction occurs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nanotechnology Reviews de Gruyter

Carbohydrate-protein interactions characterized by dual polarization hybrid plasmonic waveguide

Nanotechnology Reviews , Volume 7 (1): 8 – Feb 23, 2018

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Publisher
de Gruyter
Copyright
©2017 Walter de Gruyter GmbH, Berlin/Boston
ISSN
2191-9097
eISSN
2191-9097
DOI
10.1515/ntrev-2017-0165
Publisher site
See Article on Publisher Site

Abstract

AbstractOptical biosensors present good performance for the affinity analysis of a molecular binding event. However, most of those excited with single optical mode are “blind” to the conformational change of bound molecules. We theoretically demonstrate a dual polarization hybrid plasmonic (DPHP) waveguide with nano-slots. By addressing the structure with dual polarizations, the optogeometrical properties (density and thickness) of protein layers have been determined without ambiguity. Differences in the hybrid mode dispersion between the transverse electric (TE) and transverse magnetic (TM) modes allow to separately determine the thickness and the density at all stages during the molecular interaction. In addition, nano-slots can be equated with an effective optical capacitance resulting in a strong field confinement; thereby, subtle changes in the ambient medium can be sensed. A proof of concept is conducted by analyzing the conformational change of HepV, a recombinant fragment of collagen V, during complicated molecular interaction. Integrated with adlayer thickness and density, we can conclude that a thick sparse layer formed after heparin capture and a thin dense layer arising from HepV bound. Accordingly, HepV undergoing conformational change has been traced and verified as molecular interaction occurs.

Journal

Nanotechnology Reviewsde Gruyter

Published: Feb 23, 2018

References