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Automated high-throughput analysis of B cell spreading on immobilized antibodies with whole slide imaging

Automated high-throughput analysis of B cell spreading on immobilized antibodies with whole slide... Abstract Automated image processing methods enable objective, reproducible and high quality analysis of fluorescent cell images in a reasonable amount of time. Therefore, we propose the application of image processing pipelines based on established segmentation algorithms which can handle massive amounts of whole slide imaging data of multiple fluorescent labeled cells. After automated parameter adaption the segmentation pipelines provide high quality cell delineations revealing significant differences in the spreading of B cells: LPS-activated B cells spread significantly less on anti CD19 mAb than on anti BCR mAb and both processes could be inhibited by the F-actin destabilizing drug Cytochalasin D. Moreover, anti CD19 mAb induce a more symmetrical spreading than anti BCR mAb as reflected by the higher cell circularity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Directions in Biomedical Engineering de Gruyter

Automated high-throughput analysis of B cell spreading on immobilized antibodies with whole slide imaging

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Publisher
de Gruyter
Copyright
Copyright © 2015 by the
ISSN
2364-5504
eISSN
2364-5504
DOI
10.1515/cdbme-2015-0056
Publisher site
See Article on Publisher Site

Abstract

Abstract Automated image processing methods enable objective, reproducible and high quality analysis of fluorescent cell images in a reasonable amount of time. Therefore, we propose the application of image processing pipelines based on established segmentation algorithms which can handle massive amounts of whole slide imaging data of multiple fluorescent labeled cells. After automated parameter adaption the segmentation pipelines provide high quality cell delineations revealing significant differences in the spreading of B cells: LPS-activated B cells spread significantly less on anti CD19 mAb than on anti BCR mAb and both processes could be inhibited by the F-actin destabilizing drug Cytochalasin D. Moreover, anti CD19 mAb induce a more symmetrical spreading than anti BCR mAb as reflected by the higher cell circularity.

Journal

Current Directions in Biomedical Engineering – de Gruyter

Published: Sep 1, 2015

References

  • CellProfiler: Image Analysis Software for Identifying and Quantifying Cell Pheno-types
  • Evaluation of input modalities for the interactive image segmentation of fluorescent micrographs
  • Algorithms for cytoplasm segmentation of fluorescence labelled cells