Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/csiro-publishing/piles-of-scats-for-piles-of-dna-deriving-dna-of-lizards-from-their-TJxD0AjPD0
References (85)
-
(1999)
Isolation of microsatellite -
W. Navidi, N. Arnheim, M. Waterman (1992)
A multiple-tubes approach for accurate genotyping of very small DNA samples by using PCR: statistical considerations.American journal of human genetics, 50 2
-
(1996)
Mother–offspring recognition in two Australian lizards,Tiliqua rugosa andEgernia stokesii.AnimalBehaviour -
(2001)
Quantitative polymerase chain reaction analysis of DNA from noninvasive samples for accurate microsatellite genotyping of wild chimpanzees (Pan troglodytes verus).MolecularEcology10, 1835–1844 -
T. Broquet, E. Petit (2004)
Quantifying genotyping errors in noninvasive population geneticsMolecular Ecology, 13
-
C. Pavey, C. Burwell, C. Nano (2010)
Foraging Ecology and Habitat Use of Slater's Skink (Egernia slateri): An Endangered Australian Desert Lizard, 44
-
P. Marrero, R. Fregel, V. Cabrera, M. Nogales (2009)
Extraction of high-quality host DNA from feces and regurgitated seeds: a useful tool for vertebrate ecological studies.Biological research, 42 2
-
Michel Kohn, Robert Wayne (1997)
Facts from feces revisited.Trends in ecology & evolution, 12 6
-
Juliane Casquet, C. Thébaud, R. Gillespie (2012)
Chelex without boiling, a rapid and easy technique to obtain stable amplifiable DNA from small amounts of ethanol‐stored spidersMolecular Ecology Resources, 12
-
Louise Hebert, S. Darden, B. Pedersen, T. Dabelsteen (2010)
Increased DNA amplification success of non-invasive genetic samples by successful removal of inhibitors from faecal samples collected in the fieldConservation Genetics Resources, 3
-
Pierre Taberlet, L. Waits, Gordon Luikart (1999)
Noninvasive genetic sampling: look before you leap.Trends in ecology & evolution, 14 8
-
P. Taberlet, S. Griffin, Benoit Goossens, S. Questiau, Valérie Manceau, Nathalie Escaravage, L. Waits, Jean Bouvet (1996)
Reliable genotyping of samples with very low DNA quantities using PCR.Nucleic acids research, 24 16
-
A. Roeder, F. Archer, H. Poinar, P. Morin (2004)
A novel method for collection and preservation of faeces for genetic studiesMolecular Ecology Notes, 4
-
(2003)
J.,Roper,T. J.,Wilson,G -
L. Monteiro, Dominique Bonnemaison, A. Vekris, K. Petry, J. Bonnet, R. Vidal, J. Cabrita, F. Mégraud (1997)
Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori modelJournal of Clinical Microbiology, 35
-
A. Fenner, C. Bull (2008)
The impact of nematode parasites on the behaviour of an Australian lizard, the gidgee skink Egernia stokesiiEcological Research, 23
-
M. Piggott, A. Taylor (2003)
Extensive evaluation of faecal preservation and DNA extraction methods in Australian native and introduced speciesAustralian Journal of Zoology, 51
-
C. Bull, C. Griffin, M. Perkins (1999)
Some properties of a pheromone allowing individual recognition, from the scats of an Australian lizard, Egernia striolataacta ethologica, 2
-
(2007)
Characterisation of target nuclear DNA from faeces -
(2008)
An evaluation of non-invasive sampling -
E. Alacs, D. Alpers, P. Tores, M. Dillon, P. Spencer (2003)
Identifying the presence of quokkas (Setonix brachyurus) and other macropods using cytochrome b analyses from faecesWildlife Research, 30
-
(2013)
Evaluating the efficiency of various thermo -
A. Iyengar, A. Iyengar, V. Babu, S. Hedges, A. Venkataraman, N. Maclean, P. Morin (2005)
Phylogeography, genetic structure, and diversity in the dhole (Cuon alpinus)Molecular Ecology, 14
-
(2011)
Genetic diversity and -
R. Jones, J. Cable, M. Bruford (2008)
An evaluation of non-invasive sampling for genetic analysis in northern European reptilesHerpetological Journal, 18
-
M. Arandjelovic, Katerina Guschanski, Grit Schubert, Tara Harris, Olaf Thalmann, Heike Siedel, L. Vigilant (2009)
Two‐step multiplex polymerase chain reaction improves the speed and accuracy of genotyping using DNA from noninvasive and museum samplesMolecular Ecology Resources, 9
-
A. Beja-Pereira, Rita Oliveira, P. Alves, M. Schwartz, G. Luikart (2009)
Advancing ecological understandings through technological transformations in noninvasive geneticsMolecular Ecology Resources, 9
-
(2009)
Genotypes and nematode -
Ralner Deuter, Stephan Pietsch, Sven Hertel, Oliver Müller (1995)
A method for preparation of fecal DNA suitable for PCR.Nucleic acids research, 23 18
-
Knut Solberg, E. Bellemain, Olav Drageset, P. Taberlet, J. Swenson (2006)
An evaluation of field and non-invasive genetic methods to estimate brown bear (Ursus arctos) population sizeBiological Conservation, 128
-
(2002)
Stable aggregations in an Australian lizard, Egernia -
(2004)
Experiments in DNA extraction -
Main A. R. Bull C. M.
Mother–offspring recognition in two Australian lizards, Tiliqua rugosa and Egernia stokesii . -
C. Barrows (2006)
POPULATION DYNAMICS OF A THREATENED SAND DUNE LIZARD, 51
-
H. Watts, K. Scribner, H. García, K. Holekamp (2011)
Genetic diversity and structure in two spotted hyena populations reflects social organization and male dispersalJournal of Zoology, 285
-
Michael Panasci, W. Ballard, S. Breck, D. Rodriguez, L. Densmore, D. Wester, R. Baker (2011)
Evaluation of Fecal DNA Preservation Techniques and Effects of Sample Age and Diet on Genotyping Success, 75
-
M. Gardner, S. Cooper, C. Bull, W. Grant (1999)
Brief communication. Isolation of microsatellite loci from a social lizard, Egernia stokesii, using a modified enrichment procedureJournal of Heredity, 90
-
(2011)
Increased DNA -
Dustin Wilgers, E. Horne (2008)
Discrimination of chemical stimuli in conspecific fecal pellets by a visually adept iguanid lizard, Crotaphytus collarisJournal of Ethology, 27
-
(2002)
High levels of genetic monogamy -
A. Main, Michael Bull (1996)
Mother–offspring recognition in two Australian lizards, Tiliqua rugosa and Egernia stokesiiAnimal Behaviour, 52
-
Michael Gardner, C. Bull, Steven Cooper (2002)
High levels of genetic monogamy in the group‐living Australian lizard Egernia stokesiiMolecular Ecology, 11
-
Sharon Renan, E. Speyer, Naama Shahar, Tomer Gueta, A. Templeton, S. Bar-David (2012)
A factorial design experiment as a pilot study for noninvasive genetic samplingMolecular Ecology Resources, 12
-
Michael Gardner, C. Bull, Steven Cooper, G. Duffield (2001)
Genetic evidence for a family structure in stable social aggregations of the Australian lizard Egernia stokesiiMolecular Ecology, 10
-
(1999)
Some properties of a pheromone -
C. Vynne, Matthew Baker, Z. Breuer, Samuel Wasser (2012)
Factors influencing degradation of DNA and hormones in maned wolf scatAnimal Conservation, 15
-
Stephanie DeMay, Penny Becker, Chad Eidson, J. Rachlow, T. Johnson, L. Waits (2013)
Evaluating DNA degradation rates in faecal pellets of the endangered pygmy rabbitMolecular Ecology Resources, 13
-
(2002)
Stable aggregations in an Australian lizard , Egernia stokesii -
L. Waits, D. Paetkau (2005)
NONINVASIVE GENETIC SAMPLING TOOLS FOR WILDLIFE BIOLOGISTS: A REVIEW OF APPLICATIONS AND RECOMMENDATIONS FOR ACCURATE DATA COLLECTION, 69
-
(1982)
The distribution and abundance of the flat-tailed -
Nathaniel Valière (2002)
gimlet: a computer program for analysing genetic individual identification dataMolecular Ecology Notes, 2
-
(1995)
A method of preparation of fecal -
M. Gardner, J. Sánchez, R. Dudaniec, Leah Rheinberger, Annabel Smith, K. Saint (2008)
Tiliqua rugosa microsatellites: isolation via enrichment and characterisation of loci for multiplex PCR in T. rugosa and the endangered T. adelaidensisConservation Genetics, 9
-
C. Monroe, C. Grier, B. Kemp (2013)
Evaluating the efficacy of various thermo-stable polymerases against co-extracted PCR inhibitors in ancient DNA samples.Forensic science international, 228 1-3
-
Alain Frantz, Lisa Pope, P. Carpenter, T. Roper, Gavin Wilson, R. Delahay, Terry Burke (2003)
Reliable microsatellite genotyping of the Eurasian badger (Meles meles) using faecal DNAMolecular Ecology, 12
-
(2010)
Foraging ecology and habitat -
T. Brinkman, D. Person, M. Schwartz, K. Pilgrim, Kevin Colson, K. Hundertmark (2010)
Individual identification of Sitka black-tailed deer (Odocoileus hemionus sitkensis) using DNA from fecal pelletsConservation Genetics Resources, 2
-
Matthew Kearse, R. Moir, Amy Wilson, Steven Stones-Havas, M. Cheung, S. Sturrock, S. Buxton, A. Cooper, S. Markowitz, Chris Duran, T. Thierer, Bruce Ashton, Peter Meintjes, A. Drummond (2012)
Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence dataBioinformatics, 28
-
J. Stenglein, L. Waits, D. Ausband, P. Zager, Curt Mack (2011)
Estimating gray wolf pack size and family relationships using noninvasive genetic sampling at rendezvous sites, 92
-
(1999)
Noninvasive genetic sampling: look before -
Nathaniel Valière, C. Bonenfant, C. Toïgo, G. Luikart, J. Gaillard, F. Klein (2006)
Importance of a pilot study for non-invasive genetic sampling: genotyping errors and population size estimation in red deerConservation Genetics, 8
-
Z. Nagy (2010)
A hands-on overview of tissue preservation methods for molecular genetic analysesOrganisms Diversity & Evolution, 10
-
(2007)
Noninvasive population genetics: a review -
R. Harris, J. Winnie, S. Amish, A. Beja-Pereira, R. Godinho, V. Costa, G. Luikart (2010)
Argali Abundance in the Afghan Pamir Using Capture–Recapture Modeling From Fecal DNA, 74
-
E. Arida, Michael Bull (2008)
Optimising the design of artificial refuges for the Australian skink, Egernia stokesiiApplied Herpetology, 5
-
M. Gardner, C. Bull, A. Fenner, K. Murray, S. Donnellan (2007)
Consistent social structure within aggregations of the Australian lizard, Egernia stokesii across seven disconnected rocky outcropsJournal of Ethology, 25
-
M. Murphy, K. Kendall, Andrew Robinson, L. Waits (2007)
The impact of time and field conditions on brown bear (Ursus arctos) faecal DNA amplificationConservation Genetics, 8
-
(2007)
The impact of time -
D. Germano, Paul Smith, S. Tabor (2007)
FOOD HABITS OF THE BLUNT-NOSED LEOPARD LIZARD (GAMBELIA SILA), 52
-
Anthony Nsubuga, M. Robbins, A. Roeder, P. Morin, C. Boesch, L. Vigilant (2004)
Factors affecting the amount of genomic DNA extracted from ape faeces and the identification of an improved sample storage methodMolecular Ecology, 13
-
P. Taberlet, G. Luikart (1999)
Non-invasive genetic sampling and individual identificationBiological Journal of The Linnean Society, 68
-
(1997)
Facts from feces revisited. TRENDS in Ecology -
Elvira Lanham, C. Bull (2004)
Enhanced vigilance in groups in Egernia stokesii , a lizard with stable social aggregationsJournal of Zoology, 263
-
Annabel Smith, A. Fenner, C. Bull, M. Gardner (2009)
Genotypes and nematode infestations in an endangered lizard, Tiliqua adelaidensisApplied Herpetology, 6
-
M. Ball, R. Pither, M. Manseau, Jeffrey Clark, S. Petersen, Steven Kingston, Natasha Morrill, P. Wilson (2007)
Characterization of target nuclear DNA from faeces reduces technical issues associated with the assumptions of low-quality and quantity templateConservation Genetics, 8
-
J. Wehausen, R. Ramey, C. Epps (2004)
Experiments in DNA extraction and PCR amplification from bighorn sheep feces: the importance of DNA extraction method.The Journal of heredity, 95 6
-
Gardner M. G. Cooper S. J. B. Bull C. M. Grant W. N.
Isolation of microsatellite loci from a social lizard, Egernia stokesii , using a modified enrichment procedure. -
G. Duffield, C. Bull (1998)
SEASONAL AND ONTOGENETIC CHANGES IN THE DIET OF THE AUSTRALIAN SKINK EGERNIA STOKESIIHerpetologica, 54
-
G. Duffield, Michael Bull (2002)
Stable social aggregations in an Australian lizard, Egernia stokesiiNaturwissenschaften, 89
-
F. Turner, P. Medica (1982)
The distribution and abundance of the flat-tailed horned lizard (Phrynosoma mcallii)Copeia, 1982
-
T. Broquet, N. Ménard, E. Petit (2006)
Noninvasive population genetics: a review of sample source, diet, fragment length and microsatellite motif effects on amplification success and genotyping error ratesConservation Genetics, 8
-
M. Piggott (2004)
Effect of sample age and season of collection on the reliability of microsatellite genotyping of faecal DNAWildlife Research, 31
-
H. Cogger (1975)
Reptiles and Amphibians of Australia -
P. Morin, Karen Chambers, C. Boesch, L. Vigilant (2001)
Quantitative polymerase chain reaction analysis of DNA from noninvasive samples for accurate microsatellite genotyping of wild chimpanzees (Pan troglodytes verus)Molecular Ecology, 10
-
(2001)
Quantitative polymerase
- Publisher
- CSIRO Publishing
- Copyright
- Copyright © The Author(s). Published by CSIRO Publishing
- ISSN
- 0004-959X
- eISSN
- 1446-5698
- DOI
- 10.1071/ZO14059
- Publisher site
- See Article on Publisher Site
Abstract
Non-invasive genetic sampling using scats has a well established role in conservation biology, but has rarely been applied to reptiles. Using scats from captive and wild Egernia stokesii (Squamata, Scincidae) we evaluated two storage and six DNA-extraction methods and the reliability of subsequent genotype and sequence data. Accurate genotype and sequence data were obtained from frozen and dried captive lizard scat DNA extracted using a QIAamp DNA Stool Mini Kit and a modified Gentra Puregene method, but success rates were reduced for wild lizard scats. Wild E. stokesii eat more plants than their captive counterparts, possibly resulting in scat DNA extracts containing plant compounds that inhibit PCR-amplifications. Notably, reliable genotypes and sequences were obtained from wild E. stokesii scat DNA extracted using a Qiagen DNeasy Plant Mini Kit, a method designed to remove plant inhibitory compounds. Results highlight the opportunity for using scat-derived DNA in lizard studies, particularly for species that deposit scats in piles.
Journal
Australian Journal of Zoology – CSIRO Publishing
Published: Feb 24, 2015