Access the full text.
Sign up today, get DeepDyve free for 14 days.
D. Spurgeon, S. Hopkin (1995)
Extrapolation of the laboratory-based OECD earthworm toxicity test to metal-contaminated field sitesEcotoxicology, 4
J. Cortet, Annette Vauflery, N. Poinsot-Balaguer, L. Gomot, Christine Texier, D. Cluzeau (1999)
The use of invertebrate soil fauna in monitoring pollutant effectsEuropean Journal of Soil Biology, 35
S. Rochfort, V. Ezernieks, A. Yen (2009)
NMR-based metabolomics using earthworms as potential indicators for soil healthMetabolomics, 5
S. Craig (2004)
Betaine in human nutrition.The American journal of clinical nutrition, 80 3
Shari Jones, Mathias Jorgensen, Fatema Chowdhury, R. Rodgers, James Hartline, M. Leatham, C. Struve, K. Krogfelt, P. Cohen, T. Conway (2008)
Glycogen and Maltose Utilization by Escherichia coli O157:H7 in the Mouse IntestineInfection and Immunity, 76
Whitfield Åslund M. L. Simpson A. J. Simpson M. J.
1 H NMR metabolomics of earthworm responses to polychlorinated
S. Hellberg, M. Sjöström, S. Wold (1986)
The prediction of bradykinin potentiating potency of pentapeptides. An example of a peptide quantitative structure-activity relationship.Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry, 40 2
A. Woolf, R. Goldman, D. Bellinger (2007)
Update on the clinical management of childhood lead poisoning.Pediatric clinics of North America, 54 2
Sarah Brown, A. Simpson, M. Simpson (2009)
1H NMR metabolomics of earthworm responses to sub-lethal PAH exposureEnvironmental Chemistry, 6
C. Lin, M. Viant, R. Tjeerdema (2006)
Metabolomics: Methodologies and applications in the environmental sciencesJournal of Pesticide Science, 31
K. Kharbanda, M. Mailliard, Cheryl Baldwin, H. Beckenhauer, M. Sorrell, D. Tuma (2007)
Betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway.Journal of hepatology, 46 2
S. Verma, R. Dubey (2003)
Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plantsPlant Science, 164
K. Shah, Ritambhara Kumar, S. Verma, R. Dubey (2001)
Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedlingsPlant Science, 161
C. Callahan, M Skirazi, E. Neuhauser (1994)
Comparative toxicity of chemicals to earthwormsEnvironmental Toxicology and Chemistry, 13
M. Viant, E. Rosenblum, Ronald Tieerdema (2003)
NMR-based metabolomics: a powerful approach for characterizing the effects of environmental stressors on organism health.Environmental science & technology, 37 21
B. Beckwith-Hall, Joanne Brindle, R. Barton, M. Coen, E. Holmes, J. Nicholson, H. Antti (2002)
Application of orthogonal signal correction to minimise the effects of physical and biological variation in high resolution 1H NMR spectra of biofluids.The Analyst, 127 10
B. Somashekar, P. Kamarajan, T. Danciu, Y. Kapila, A. Chinnaiyan, Thekkelnaycke Rajendiran, A. Ramamoorthy (2011)
Magic angle spinning NMR-based metabolic profiling of head and neck squamous cell carcinoma tissues.Journal of proteome research, 10 11
Q. Guo, Jasmin Sidhu, Timothy Ebbels, F. Rana, David Spurgeon, C. Svendsen, S. Stürzenbaum, P. Kille, A. Morgan, Jacob Bundy (2009)
Validation of metabolomics for toxic mechanism of action screening with the earthworm Lumbricus rubellusMetabolomics, 5
V. Sivapiriya, Jayanthisakthisekaran, S. Venkatraman (2006)
Effects of dimethoate (O,O-dimethyl S-methyl carbamoyl methyl phosphorodithioate) and Ethanol in antioxidant status of liver and kidney of experimental micePesticide Biochemistry and Physiology, 85
R. Ansari, R. Shukla, R. Yadav, K. Seth, A. Pant, Dhirendra Singh, A. Agrawal, F. Islam, V. Khanna (2012)
Cholinergic Dysfunctions and Enhanced Oxidative Stress in the Neurobehavioral Toxicity of Lambda-Cyhalothrin in Developing RatsNeurotoxicity Research, 22
J. Bundy, D. Spurgeon, C. Svendsen, P. Hankard, J. Weeks, D. Osborn, J. Lindon, J. Nicholson (2004)
Environmental Metabonomics: Applying Combination Biomarker Analysis in Earthworms at a Metal Contaminated SiteEcotoxicology, 13
O. Beckonert, H. Keun, T. Ebbels, J. Bundy, E. Holmes, J. Lindon, J. Nicholson (2007)
Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extractsNature Protocols, 2
P. Skubała, T. Zaleski (2012)
Heavy metal sensitivity and bioconcentration in oribatid mites (Acari, Oribatida) Gradient study in meadow ecosystems.The Science of the total environment, 414
A. Weljie, R. Dowlatabadi, B. Miller, Hans Vogel, F. Jirik (2007)
An inflammatory arthritis-associated metabolite biomarker pattern revealed by 1H NMR spectroscopy.Journal of proteome research, 6 9
M. Simpson, J. McKelvie (2009)
Environmental metabolomics: new insights into earthworm ecotoxicity and contaminant bioavailability in soilAnalytical and Bioanalytical Chemistry, 394
E. Neuhauser, R. Loehr, D. Milligan, M. Małecki (1985)
Toxicity of metals to the earthworm Eisenia fetidaBiology and Fertility of Soils, 1
J. Xia, D. Wishart (2010)
MetPA: a web-based metabolomics tool for pathway analysis and visualizationBioinformatics, 26 18
H. Hacker, P. Bannasch, A. Columbano (1990)
Effect of lead nitrate on liver carbohydrate enzymes and glycogen content in the rat.Carcinogenesis, 11 12
J. Yuk, M. Simpson, A. Simpson (2012)
Coelomic fluid: a complimentary biological medium to assess sub-lethal endosulfan exposure using 1H NMR-based earthworm metabolomicsEcotoxicology, 21
C. Xiao, Yan Gu, Chunyi Zhou, Lang Wang, Min-min Zhang, D. Ruan (2006)
Pb2+ impairs GABAergic synaptic transmission in rat hippocampal slices: A possible involvement of presynaptic calcium channelsBrain Research, 1088
J. Bundy, E. Lenz, N. Bailey, C. Gavaghan, C. Svendsen, D. Spurgeon, P. Hankard, D. Osborn, J. Weeks, S. Trauger, Paul Speir, Ian Sanders, J. Lindon, J. Nicholson, Huiru Tang (2002)
Metabonomic assessment of toxicity of 4‐fluoroaniline, 3,5‐difluoroaniline and 2‐fluoro‐4‐methylaniline to the earthworm Eisenia veneta (rosa): Identification of new endogenous biomarkersEnvironmental Toxicology and Chemistry, 21
R. Franco, R. Sánchez-Olea, E. Reyes-Reyes, M. Panayiotidis (2009)
Environmental toxicity, oxidative stress and apoptosis: ménage à trois.Mutation research, 674 1-2
M. Mench, V. Didier, M. Löffler, A. Gomez, P. Masson (1994)
A mimicked in-situ remediation study of metal-contaminated soils with emphasis on cadmium and leadJournal of Environmental Quality, 23
Jacob Bundy, Hector Keun, Jasmin Sidhu, David Spurgeon, C. Svendsen, P. Kille, A. Morgan (2007)
Metabolic profile biomarkers of metal contamination in a sentinel terrestrial species are applicable across multiple sites.Environmental science & technology, 41 12
B. Ganesan, S. Buddhan, R. Anandan, R. Sivakumar, R. AnbinEzhilan (2010)
Antioxidant defense of betaine against isoprenaline-induced myocardial infarction in ratsMolecular Biology Reports, 37
E. Lenz, Jason Weeks, J. Lindon, Daniel Osborn, J. Nicholson (2005)
Qualitative high field 1H-NMR spectroscopy for the characterization of endogenous metabolites in earthworms with biochemical biomarker potentialMetabolomics, 1
Paul Anderson, D. Mahle, T. Doom, N. Reo, N. DelRaso, M. Raymer (2011)
Dynamic adaptive binning: an improved quantification technique for NMR spectroscopic dataMetabolomics, 7
B. Majer, D. Tscherko, A. Paschke, R. Wennrich, M. Kundi, E. Kandeler, S. Knasmüller (2002)
Effects of heavy metal contamination of soils on micronucleus induction in Tradescantia and on microbial enzyme activities: a comparative investigation.Mutation research, 515 1-2
M. Viant (2008)
Recent developments in environmental metabolomics.Molecular bioSystems, 4 10
David Spurgeon, S. Hopkin, D. Jones (1994)
Effects of cadmium, copper, lead and zinc on growth, reproduction and survival of the earthworm Eisenia fetida (Savigny): Assessing the environmental impact of point-source metal contamination in terrestrial ecosystems.Environmental pollution, 84 2
F. Dieterle, A. Ross, G. Schlotterbeck, H. Senn (2006)
Probabilistic quotient normalization as robust method to account for dilution of complex biological mixtures. Application in 1H NMR metabonomics.Analytical chemistry, 78 13
S. Khan, Qing Cao, Ying-juan Zheng, Youda Huang, Yong-guan Zhu (2008)
Health risks of heavy metals in contaminated soils and food crops irrigated with wastewater in Beijing, China.Environmental pollution, 152 3
C. Svendsen, D. Spurgeon, P. Hankard, J. Weeks (2004)
A review of lysosomal membrane stability measured by neutral red retention: is it a workable earthworm biomarker?Ecotoxicology and environmental safety, 57 1
Yuanhu Shao, Weixin Zhang, J. Shen, Lixia Zhou, H. Xia, W. Shu, H. Ferris, S. Fu (2008)
Nematodes as indicators of soil recovery in tailings of a lead/zinc mineSoil Biology & Biochemistry, 40
Brian Lankadurai, D. Wolfe, Melissa Åslund, A. Simpson, M. Simpson (2013)
1H NMR-based metabolomic analysis of polar and non-polar earthworm metabolites after sub-lethal exposure to phenanthreneMetabolomics, 9
G. Flora, D. Gupta, A. Tiwari (2012)
Toxicity of lead: A review with recent updatesInterdisciplinary Toxicology, 5
S. Dubey, P. Misra, S. Dwivedi, S. Chatterjee, S. Bag, Shrikant Mantri, M. Asif, A. Rai, Smita Kumar, M. Shri, P. Tripathi, R. Tripathi, P. Trivedi, D. Chakrabarty, R. Tuli (2010)
Transcriptomic and metabolomic shifts in rice roots in response to Cr (VI) stressBMC Genomics, 11
Taotao Wei, Y. Ni, Jingwu Hou, Chang Chen, Baolu Zhao, Wenjuan Xin (2000)
Hydrogen peroxide-induced oxidative damage and apoptosis in cerebellar granule cells: protection by Ginkgo biloba extract.Pharmacological research, 41 4
S. Lasley, M. Gilbert (2002)
Rat hippocampal glutamate and GABA release exhibit biphasic effects as a function of chronic lead exposure level.Toxicological sciences : an official journal of the Society of Toxicology, 66 1
J. McKelvie, J. Yuk, Yunping Xu, A. Simpson, M. Simpson (2009)
1H NMR and GC/MS metabolomics of earthworm responses to sub-lethal DDT and endosulfan exposureMetabolomics, 5
Huifeng Wu, A. Southam, A. Hines, M. Viant (2008)
High-throughput tissue extraction protocol for NMR- and MS-based metabolomics.Analytical biochemistry, 372 2
J. Bundy, J. Sidhu, F. Rana, D. Spurgeon, C. Svendsen, J. Wren, J. Wren, S. Stürzenbaum, A. Morgan, P. Kille (2008)
'Systems toxicology' approach identifies coordinated metabolic responses to copper in a terrestrial non-model invertebrate, the earthworm Lumbricus rubellusBMC Biology, 6
R. Tibshirani (1996)
Regression Shrinkage and Selection via the LassoJournal of the royal statistical society series b-methodological, 58
I. Baranowska-Bosiacka, I. Gutowska, C. Marchetti, M. Rutkowska, M. Marchlewicz, A. Kolasa, A. Prokopowicz, I. Wiernicki, K. Piotrowska, M. Baśkiewicz, K. Safranow, B. Wiszniewska, D. Chlubek (2011)
Altered energy status of primary cerebellar granule neuronal cultures from rats exposed to lead in the pre- and neonatal period.Toxicology, 280 1-2
A. Shafy, V. Molinie, M. Cortès-Morichetti, V. Hupertan, N. Lila, J. Chachques (2012)
Comparison of the Effects of Adenosine, Inosine, and Their Combination as an Adjunct to Reperfusion in the Treatment of Acute Myocardial InfarctionISRN Cardiology, 2012
Brian Lankadurai, D. Wolfe, A. Simpson, M. Simpson (2011)
1H NMR-based metabolomic observation of a two-phased toxic mode of action in Eisenia fetida after sub-lethal phenanthrene exposureEnvironmental Chemistry, 8
Sarah Brown, A. Simpson, M. Simpson (2008)
Evaluation of sample preparation methods for nuclear magnetic resonance metabolic profiling studies with Eisenia fetidaEnvironmental Toxicology and Chemistry, 27
Melissa Åslund, A. Simpson, M. Simpson (2011)
1H NMR metabolomics of earthworm responses to polychlorinated biphenyl (PCB) exposure in soilEcotoxicology, 20
J. Klaunig, Yong Xu, J. Isenberg, S. Bachowski, K. Kolaja, Jiazhong Jiang, D. Stevenson, E. Walborg (1998)
The role of oxidative stress in chemical carcinogenesis.Environmental Health Perspectives, 106
Y. Hochberg, Y. Benjamini (1990)
More powerful procedures for multiple significance testing.Statistics in medicine, 9 7
Y. Benjamini, Y. Hochberg (1995)
Controlling the false discovery rate: a practical and powerful approach to multiple testingJournal of the royal statistical society series b-methodological, 57
S. Suthar, S. Singh, S. Dhawan (2008)
Earthworms as bioindicator of metals (Zn, Fe, Mn, Cu, Pb and Cd) in soils: Is metal bioaccumulation affected by their ecological category?Ecological Engineering, 32
Environmental contextHeavy metals are non-degradable and are therefore a severe and persistent environmental menace. The toxic effects of Pb were investigated using NMR to determine the metabolic changes in earthworms exposed to Pb. The approach using 1H NMR to analyse earthworm metabolomics demonstrated great potential as a reliable, rapid and convenient tool to assess the toxicity of heavy metals and could be used to identify warning signs of heavy metal contamination of soil.AbstractA 1H nuclear magnetic resonance (NMR)-based approach to metabolomics combined with atomic absorption spectroscopy, histopathological examination and biochemical assessment was used to determine the toxic effects of lead (Pb) on earthworms (Eisenia fetida). Earthworms were exposed to Pb in a lead nitrate solution at converted concentrations of 1.25, 5.0 and 20gcm2 in contact tests for 48h. Based on histopathological inspection, the epidermis, muscles, chloragogenous tissues and intestinal epithelium were severely impaired. Based on biochemical assessment, a disruption of the antioxidative system and neurotoxic effects in earthworms occurred following exposure to Pb. Orthogonal signal correctionpartial least-squares-discriminant analysis of NMR profiles indicated that Pb exposure in earthworms caused widespread metabolic changes, which were associated with oxidative stress, neurotransmitter imbalance, disruption of osmotic equilibrium and interference in energy metabolism and nucleic acid metabolism. The integrated metabolomics approach provided new insights into Pb-induced toxicity in earthworms. Metabolomics is a powerful and highly effective approach and has great promise to determine the ecotoxicological effects and the underlying mechanisms of toxicity of heavy metals.
Environmental Chemistry – CSIRO Publishing
Published: Mar 23, 2016
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.