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Fifteen microsatellite loci for use in non-invasive sampling studies of the antilopine wallaroo (Macropus antilopinus)

Fifteen microsatellite loci for use in non-invasive sampling studies of the antilopine wallaroo... A set of 15 microsatellite loci was optimised for multilocus genotyping of non-invasively collected samples of Macropus antilopinus (antilopine wallaroo). Primers were combined in three PCR multiplexes in order to increase the quality of genotypes from scat samples and to allow for replication. In a screen of 104 scat samples from two populations in north-eastern Australia, three loci were found to be monomorphic while the remaining 12 loci had 210 alleles. Genotype frequencies for all 12 microsatellite loci from the two populations did not differ significantly from HardyWeinberg equilibrium, and there was no evidence of linkage disequilibrium. These informative markers are specifically designed for non-invasive samples and will be used to assess population structure and conservation genetics of this species in the future. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Australian Journal of Zoology CSIRO Publishing

Fifteen microsatellite loci for use in non-invasive sampling studies of the antilopine wallaroo (Macropus antilopinus)

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Publisher
CSIRO Publishing
Copyright
Copyright © The Author(s). Published by CSIRO Publishing
ISSN
0004-959X
eISSN
1446-5698
DOI
10.1071/ZO13074
Publisher site
See Article on Publisher Site

Abstract

A set of 15 microsatellite loci was optimised for multilocus genotyping of non-invasively collected samples of Macropus antilopinus (antilopine wallaroo). Primers were combined in three PCR multiplexes in order to increase the quality of genotypes from scat samples and to allow for replication. In a screen of 104 scat samples from two populations in north-eastern Australia, three loci were found to be monomorphic while the remaining 12 loci had 210 alleles. Genotype frequencies for all 12 microsatellite loci from the two populations did not differ significantly from HardyWeinberg equilibrium, and there was no evidence of linkage disequilibrium. These informative markers are specifically designed for non-invasive samples and will be used to assess population structure and conservation genetics of this species in the future.

Journal

Australian Journal of ZoologyCSIRO Publishing

Published: Dec 13, 2013

References