Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

A novel method to determine trimethylantimony concentrations in plant tissue

A novel method to determine trimethylantimony concentrations in plant tissue Environmental contextAntimony enters the soil mostly through mining and shooting activities and can thereafter be taken up by plants. In the soil, antimony may undergo several transformations such as biomethylation, leading to the formation of trimethylantimony. Here, we measured for the first time the uptake and translocation of trimethylantimony in a plant using a new extraction and analysis method.AbstractAntimony (Sb) is a relevant pollutant that can be found in elevated concentrations in soils near Sb mines and at shooting ranges. In soils, Sb occurs as trivalent Sb, SbIII, pentavalent Sb, SbV, or trimethylantimony, TMSb ((CH3)3SbO), the latter being the result of microbial biomethylation. It is important to understand the transfer of Sb species from soil to plants to assess the role of Sb in the food chain. However, this research has historically been hampered by the lack of suitable extraction and analytical methods. In this study, we validated an efficient and reliable extraction technique using oxalic acid and ascorbic acid (72.61.3 of Sb extracted) as well as a high-pressure liquid chromatographyinductively coupled plasma mass spectrometry (HPLC-ICP-MS) speciation analysis method to assess the uptake of TMSb in ryegrass (Lolium perenne L.), a common pasture plant, in a hydroponics experiment. Our results show that TMSb and SbIII are not converted to other species during extraction and that TMSb is taken up by ryegrass roots and translocated to the shoots. Our study also points at specific methylationdemethylation mechanisms occurring in the plant. Moreover, an unknown Sb species was found in the shoots of TMSb-treated plants, highlighting the need for further research. These new extraction and speciation methods will enable researchers to study the soilplant transfer of organo-Sb compounds in a reliable and consistent manner. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Environmental Chemistry CSIRO Publishing

A novel method to determine trimethylantimony concentrations in plant tissue

Download PDF
8 pages

Loading next page...
 
/lp/csiro-publishing/a-novel-method-to-determine-trimethylantimony-concentrations-in-plant-DdIe5S0bhP

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
CSIRO Publishing
Copyright
Copyright © The Author(s). Published by CSIRO Publishing
ISSN
1448-2517
eISSN
1449-8979
DOI
10.1071/EN16018
Publisher site
See Article on Publisher Site

Abstract

Environmental contextAntimony enters the soil mostly through mining and shooting activities and can thereafter be taken up by plants. In the soil, antimony may undergo several transformations such as biomethylation, leading to the formation of trimethylantimony. Here, we measured for the first time the uptake and translocation of trimethylantimony in a plant using a new extraction and analysis method.AbstractAntimony (Sb) is a relevant pollutant that can be found in elevated concentrations in soils near Sb mines and at shooting ranges. In soils, Sb occurs as trivalent Sb, SbIII, pentavalent Sb, SbV, or trimethylantimony, TMSb ((CH3)3SbO), the latter being the result of microbial biomethylation. It is important to understand the transfer of Sb species from soil to plants to assess the role of Sb in the food chain. However, this research has historically been hampered by the lack of suitable extraction and analytical methods. In this study, we validated an efficient and reliable extraction technique using oxalic acid and ascorbic acid (72.61.3 of Sb extracted) as well as a high-pressure liquid chromatographyinductively coupled plasma mass spectrometry (HPLC-ICP-MS) speciation analysis method to assess the uptake of TMSb in ryegrass (Lolium perenne L.), a common pasture plant, in a hydroponics experiment. Our results show that TMSb and SbIII are not converted to other species during extraction and that TMSb is taken up by ryegrass roots and translocated to the shoots. Our study also points at specific methylationdemethylation mechanisms occurring in the plant. Moreover, an unknown Sb species was found in the shoots of TMSb-treated plants, highlighting the need for further research. These new extraction and speciation methods will enable researchers to study the soilplant transfer of organo-Sb compounds in a reliable and consistent manner.

Journal

Environmental Chemistry – CSIRO Publishing

Published: Jan 1, 2016

References

  • Antimony sinks in the weathering crust of bullets from Swiss shooting ranges.
    Ackermann S. Gieré R. Newville M. Majzlan J.
  • Distribution, speciation and availability of
    Okkenhaug G. Zhu Y. Luo L. Lei M. Li X. Mulder J.
  • Antimony in the soil–water–plant system at the Su Suergiu abandoned mine (Sardinia, Italy): strategies to mitigate contamination.
    Cidu R. Biddau R. Dore E. Vacca A. Marini L.
  • Mobility of antimony, arsenic and lead at a former antimony mine, Glendinning, Scotland.
    Macgregor K. MacKinnon G. Farmer J. G. Graham M. C.
  • Comparison of antimony behavior with that of arsenic under various soil redox conditions.
    Mitsunobu S. Harada T. Takahashi Y.
  • Speciation of antimony and arsenic in the soils and plants in an old antimony mine.
    Wei C. Ge Z. Chu W. Feng R.
  • Antimony accumulation and toxicity tolerance mechanisms in Trifolium species.
    Corrales I. Barceló J. Bech J. Poschenrieder C.
  • Antimony uptake, translocation and speciation in rice plants exposed to antimonite and antimonate.
    Ren J. Ma L. Q. Sun H. Cai F. Luo J.
  • Effect of iron plaque on antimony uptake by rice ( Oryza sativa L.).
    Cui X. Wang Y. Hockmann K. Zhou D.
  • Uptake, translocation and transformation of antimony in rice ( Oryza sativa L.) seedlings.
    Cai F. Ren J. Tao S. Wang X.
  • Effects of waterlogging on the solubility and redox state of Sb in a shooting range soil and its uptake by grasses: a tank experiment.
    Wan X. Tandy S. Hockmann K. Schulin R.
  • Alkyl derivatives of antimony in the environment.
    Filella M.
  • Organoarsenicals. Uptake, metabolism, and toxicity.
    Dopp E. Kligerman A. D. Diaz-Bone R. A.
  • Heavy metal toxicity, part I: arsenic and mercury.
    Graeme K. A. Pollack C. V. Jr
  • Antimony bioavailability: knowledge and research perspectives for sustainable agricultures.
    Pierart A. Shahid M. Sejalon-Delmas N. Dumat C.
  • Microbial methylation of metalloids: arsenic, antimony, and bismuth.
    Bentley R. Chasteen T. G.
  • Adsorption of methylantimony and methylarsenic on soils, sediments, and mine tailings from antimony mine area.
    Yang H. He M.
  • Simultaneous determination of inorganic and organic antimony species by using anion-exchange phases for HPLC-ICP-MS and their application to plant extracts of Pteris vittata .
    Müller K. Daus B. Mattusch J. Staerk H. Wennrich R.
  • An analytical method for the detection of methylantimony species in environmental matrices: methylantimony levels in some UK plant material.
    Craig P. Forster S. Jenkins R. Miller D.
  • Antimony speciation in terrestrial plants. Comparative studies on extraction methods.
    Miravet R. Bonilla E. Lopez-Sanchez J. F. Rubio R.
  • Speciation of antimony in environmental matrices by coupled techniques.
    Miravet R. Hernandez-Nataren E. Sahuquillo A. Rubio R. Lopez-Sanchez J. F.
  • Speciation of antimony(III), antimony(V) and trimethylstiboxide by ion chromatography with inductively coupled plasma atomic emission spectrometric and mass spectrometric detection.
    Ulrich N.
  • Developments with anion-exchange stationary phases for HPLC-ICP-MS analysis of antimony species.
    Nash M. J. Maskall J. E. Hill S. J.
  • Optimization of an HPLC-HG-AFS method for screening Sb V , Sb III , and Me 3 SbBr 2 in water samples.
    Sayago A. Beltran R. Recamales M. A. F. Gomez-Ariza J. L.
  • HPLC-HG-ICP-MS: a sensitive and selective method for inorganic arsenic in seafood.
    Pétursdóttir A. H. Gunnlaugsdottir H. Joerundsdottir H. Mestrot A. Krupp E. M. Feldmann J.
  • Achieving 100 % efficient post-column hydride generation for As speciation analysis by atomic fluorescence spectrometry.
    Marschner K. Musil S. Dëdina J.
  • Antimony speciation analysis in sediment reference materials using high-performance liquid chromatography coupled to hydride generation atomic fluorescence spectrometry.
    Potin-Gautier M. Pannier F. Quiroz W. Pinochet H. de Gregori I.
  • Simultaneous analysis of Sb III , Sb V and TMSb by high-performance liquid chromatography–inductively coupled plasma–mass spectrometry detection: application to antimony speciation in soil samples.
    Ge Z. Wei C.
  • Development and validation of the new IPA microwave-assisted beach method 3051A.
    Link D. D. Walter P. J. Kingston H. M.
  • New considerations about the separation and quantification of antimony species by ion chromatography–hydride generation atomic fluorescence spectrometry.
    Miravet R. López-Sánchez J. F. Rubio R.
  • Carbon-enhanced inductively coupled plasma mass spectrometric detection of arsenic and selenium and its application to arsenic speciation.
    Larsen E. H. Sturup S.
  • Influence of iron plaque and cultivars on antimony uptake by and translocation in rice ( Oryza sativa L.) seedlings exposed to Sb III or Sb V .
    Huang Y. Chen Z. Liu W.
  • Structural evidence of the similarity of Sb(OH) 3 and As(OH) 3 with glycerol: implications for their uptake.
    Porquet A. Filella M.
  • Arabidopsis NIP1;1 transports antimonite and determines antimonite sensitivity.
    Kamiya T. Fujiwara T.
  • Uptake of antimonite and antimonate by arsenic hyperaccumulator Pteris vittata : effects of chemical analogs and transporter inhibitor.
    Tisarum R. Chen Y. Dong X. Lessl J. T. Ma L. Q.
  • Methylated arsenic species in plants originate from soil microorganisms.
    Lomax C. Liu W. Wu L. Xue K. Xiong J. Zhou J. McGrath S. P. Meharg A. A. Miller A. J. Zhao F.