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Practicality of detection of genetically modified organisms (GMOs) in food.

Practicality of detection of genetically modified organisms (GMOs) in food. The increase in new GMOs (genetically modified organisms) has led to specific legislation world-wide to regulate the presence and/or the amount of GMOs in food, food ingredients, feeds and agriculture commodities. Regulatory demands of labelling and traceability of GMOs in the food chain need suitable sampling protocols and analytical methods. At present, two main techniques, the DNA-based polymerase chain reaction (PCR) and the protein-based enzyme-linked immunosorbent assay (ELISA) are most widely used, although new technologies, such as microarrays, have been also developed. The highly specific and sensitive real-time PCR (RT-PCR) is the method of choice for GMO quantitative estimation. The potential and the limits of PCR and the practicality of RT-PCR for GMO detection are discussed in the light of actual legislation and of the constraints imposed by the food production chain. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AgBiotechNet Centre for Agricultural Bioscience International

Practicality of detection of genetically modified organisms (GMOs) in food.

Rizzi, A.; Sorlini, C.; Daffonchio, D.
AgBiotechNet , Volume 6 (ABN 130) – Nov 3, 2004
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Publisher
Centre for Agricultural Bioscience International
Copyright
©2011 CAB International
ISSN
2304-3075

Abstract

The increase in new GMOs (genetically modified organisms) has led to specific legislation world-wide to regulate the presence and/or the amount of GMOs in food, food ingredients, feeds and agriculture commodities. Regulatory demands of labelling and traceability of GMOs in the food chain need suitable sampling protocols and analytical methods. At present, two main techniques, the DNA-based polymerase chain reaction (PCR) and the protein-based enzyme-linked immunosorbent assay (ELISA) are most widely used, although new technologies, such as microarrays, have been also developed. The highly specific and sensitive real-time PCR (RT-PCR) is the method of choice for GMO quantitative estimation. The potential and the limits of PCR and the practicality of RT-PCR for GMO detection are discussed in the light of actual legislation and of the constraints imposed by the food production chain.

Journal

AgBiotechNet – Centre for Agricultural Bioscience International

Published: Nov 3, 2004

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