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Diagnosis of the strongyloid nematode Strongyloides venezuelensis in experimentally infected rats

Diagnosis of the strongyloid nematode Strongyloides venezuelensis in experimentally infected rats Abstract Strongyloides venezuelensis is an intestinal nematode of rats, frequently used as a model for studying human and animal strongyloidiasis. In the present study, we evaluated parasitological, serological and molecular methods for the diagnosis of experimental S. venezuelensis in rats, Rattus norvegicus. Blood and faecal samples were collected and analysed up to 60 days post infection (pi) with adult worm recovery occurring from 5 to 45 days pi. Using an enzyme-linked immunosorbent assay (ELISA), serum levels of IgG antibodies increased up to 28 days pi, thereafter decreasing by day 60 pi. Polymerase chain reaction (PCR) assays detected S. venezuelensis DNA in faecal samples of rats from 5 to 21 days pi. The present study therefore represents the first step towards improving the diagnosis of experimental strongyloidiasis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Helminthology Cambridge University Press

Diagnosis of the strongyloid nematode Strongyloides venezuelensis in experimentally infected rats

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References (27)

Publisher
Cambridge University Press
Copyright
Copyright © Cambridge University Press 2015 
ISSN
1475-2697
eISSN
0022-149X
DOI
10.1017/S0022149X15000528
Publisher site
See Article on Publisher Site

Abstract

Abstract Strongyloides venezuelensis is an intestinal nematode of rats, frequently used as a model for studying human and animal strongyloidiasis. In the present study, we evaluated parasitological, serological and molecular methods for the diagnosis of experimental S. venezuelensis in rats, Rattus norvegicus. Blood and faecal samples were collected and analysed up to 60 days post infection (pi) with adult worm recovery occurring from 5 to 45 days pi. Using an enzyme-linked immunosorbent assay (ELISA), serum levels of IgG antibodies increased up to 28 days pi, thereafter decreasing by day 60 pi. Polymerase chain reaction (PCR) assays detected S. venezuelensis DNA in faecal samples of rats from 5 to 21 days pi. The present study therefore represents the first step towards improving the diagnosis of experimental strongyloidiasis.

Journal

Journal of HelminthologyCambridge University Press

Published: Jul 14, 2015

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