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In Vitro Analysis of Murine B-Cell Development

In Vitro Analysis of Murine B-Cell Development The development of B lymphocytes provides a useful model system for the examination of factors that regulate growth and differentiation. The wealth of information on the molecular biology of immunoglobulin gene structure and expression (1, 2) coupled with the abundant supply of tumor models for various stages of B-cell development (3, 4, 5, 6) serves as a framework for studies on normal or cultured cell populations. The structure and expression of immunoglobulin genes at the DNA, RNA, and protein levels can be measured with great accuracy and discrimination. Many additional phenotypic markers, including other cell-surface antigens, can be similarly followed. Using such markers one can assess the developmental phenotype of a cell population or clonal cell lines before and after growth alterations. In order to examine such changes in detail, researchers needed to develop in vitro systems to grow B cells and their precursors. The purpose of this review is to compare the range of in vitro assay and culture systems useful to evaluate different stages of B-cell development. A maj or future goal of this experimental approach is to reconstruct in vitro the entire pathway of B-cell development and functional responses. Special emphasis will be placed on http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annual Review of Immunology Annual Reviews

In Vitro Analysis of Murine B-Cell Development

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Publisher
Annual Reviews
Copyright
Copyright 1985 Annual Reviews. All rights reserved
Subject
Review Articles
ISSN
0732-0582
eISSN
1545-3278
DOI
10.1146/annurev.iy.03.040185.001241
pmid
3904770
Publisher site
See Article on Publisher Site

Abstract

The development of B lymphocytes provides a useful model system for the examination of factors that regulate growth and differentiation. The wealth of information on the molecular biology of immunoglobulin gene structure and expression (1, 2) coupled with the abundant supply of tumor models for various stages of B-cell development (3, 4, 5, 6) serves as a framework for studies on normal or cultured cell populations. The structure and expression of immunoglobulin genes at the DNA, RNA, and protein levels can be measured with great accuracy and discrimination. Many additional phenotypic markers, including other cell-surface antigens, can be similarly followed. Using such markers one can assess the developmental phenotype of a cell population or clonal cell lines before and after growth alterations. In order to examine such changes in detail, researchers needed to develop in vitro systems to grow B cells and their precursors. The purpose of this review is to compare the range of in vitro assay and culture systems useful to evaluate different stages of B-cell development. A maj or future goal of this experimental approach is to reconstruct in vitro the entire pathway of B-cell development and functional responses. Special emphasis will be placed on

Journal

Annual Review of Immunology – Annual Reviews

Published: Apr 1, 1985

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