Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Molecular karyotype analysis of Cryptosporidium parvum: evidence for eight chromosomes and a low-molecular-size molecule

Molecular karyotype analysis of Cryptosporidium parvum: evidence for eight chromosomes and a... DS Blunt, NV Khramtsov, SJ Upton and BA Montelone Division of Biology, Kansas State University, Manhattan 66506-4901, USA. We report improved separation of chromosome-sized DNA molecules of the coccidian parasite Cryptosporidium parvum with contour-clamped homogeneous electric fields (CHEF). We used scanning densitometry to determine that the most likely number of chromosomes is eight. Molecular probes consisting of cloned genes were used to distinguish each of five bands visible on CHEF gels. We have also identified a low- molecular-size DNA molecule possibly related to the 35-kb circular DNAs found in other Apicomplexa. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical and Vaccine Immunology American Society For Microbiology

Molecular karyotype analysis of Cryptosporidium parvum: evidence for eight chromosomes and a low-molecular-size molecule

Download PDF

Molecular karyotype analysis of Cryptosporidium parvum: evidence for eight chromosomes and a low-molecular-size molecule

Blunt, DS; Khramtsov, NV; Upton, SJ; Montelone, BA
Clinical and Vaccine Immunology , Volume 4 (1): 11 – Jan 1, 1997

Abstract

DS Blunt, NV Khramtsov, SJ Upton and BA Montelone Division of Biology, Kansas State University, Manhattan 66506-4901, USA. We report improved separation of chromosome-sized DNA molecules of the coccidian parasite Cryptosporidium parvum with contour-clamped homogeneous electric fields (CHEF). We used scanning densitometry to determine that the most likely number of chromosomes is eight. Molecular probes consisting of cloned genes were used to distinguish each of five bands visible on CHEF gels. We have also identified a low- molecular-size DNA molecule possibly related to the 35-kb circular DNAs found in other Apicomplexa.

Loading next page...
 
/lp/american-society-for-microbiology/molecular-karyotype-analysis-of-cryptosporidium-parvum-evidence-for-a86yZ9wOrc

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
American Society For Microbiology
Copyright
Copyright © 1997 by the American Society For Microbiology.
ISSN
1556-6811
eISSN
1556-6811
Publisher site
See Article on Publisher Site

Abstract

DS Blunt, NV Khramtsov, SJ Upton and BA Montelone Division of Biology, Kansas State University, Manhattan 66506-4901, USA. We report improved separation of chromosome-sized DNA molecules of the coccidian parasite Cryptosporidium parvum with contour-clamped homogeneous electric fields (CHEF). We used scanning densitometry to determine that the most likely number of chromosomes is eight. Molecular probes consisting of cloned genes were used to distinguish each of five bands visible on CHEF gels. We have also identified a low- molecular-size DNA molecule possibly related to the 35-kb circular DNAs found in other Apicomplexa.

Journal

Clinical and Vaccine Immunology – American Society For Microbiology

Published: Jan 1, 1997

There are no references for this article.