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Comparison of Three Different Methods for Measuring Classical Pathway Complement Activity

Comparison of Three Different Methods for Measuring Classical Pathway Complement Activity The complement system plays an important role in host defense against infection and in most inflammatory processes. The standard 50% hemolytic complement (CH 50 ) assay is the most commonly used method of screening patient sera for functional activity of the classical complement pathway. Our objective in this study was to compare two newer methods (the enzyme immunoassay and the liposome immunoassay) to a commercial CH 50 assay for measuring total classical complement activity. We conclude that both newer methods compare well with a CH 50 assay and are equally sensitive in screening routine clinical sera. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical and Vaccine Immunology American Society For Microbiology

Comparison of Three Different Methods for Measuring Classical Pathway Complement Activity

Comparison of Three Different Methods for Measuring Classical Pathway Complement Activity

Clinical and Vaccine Immunology , Volume 6 (1): 137 – Jan 1, 1999

Abstract

The complement system plays an important role in host defense against infection and in most inflammatory processes. The standard 50% hemolytic complement (CH 50 ) assay is the most commonly used method of screening patient sera for functional activity of the classical complement pathway. Our objective in this study was to compare two newer methods (the enzyme immunoassay and the liposome immunoassay) to a commercial CH 50 assay for measuring total classical complement activity. We conclude that both newer methods compare well with a CH 50 assay and are equally sensitive in screening routine clinical sera.

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Publisher
American Society For Microbiology
Copyright
Copyright © 1999 by the American Society For Microbiology.
ISSN
1556-6811
eISSN
1556-6811
Publisher site
See Article on Publisher Site

Abstract

The complement system plays an important role in host defense against infection and in most inflammatory processes. The standard 50% hemolytic complement (CH 50 ) assay is the most commonly used method of screening patient sera for functional activity of the classical complement pathway. Our objective in this study was to compare two newer methods (the enzyme immunoassay and the liposome immunoassay) to a commercial CH 50 assay for measuring total classical complement activity. We conclude that both newer methods compare well with a CH 50 assay and are equally sensitive in screening routine clinical sera.

Journal

Clinical and Vaccine ImmunologyAmerican Society For Microbiology

Published: Jan 1, 1999

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