Comparison of Enzyme Immunoassays for Detection of Antibodies to Hepatitis D Virus in Serum
Abstract
Comparison of Enzyme Immunoassays for Detection of Antibodies to Hepatitis D Virus in Serum Siu-Kei Chow a * , Ederlyn E. Atienza a , Linda Cook a , Harry Prince b * , Patricia Slev c , Mary Lapé-Nixon b and Keith R. Jerome a , d a Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA b Focus Diagnostics Reference Laboratory, San Juan Capistrano, California, USA c Department of Pathology, University of Utah, Salt Lake City, Utah, USA d The Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA R. L. Hodinka , Editor University of South Carolina School of Medicine Greenville ABSTRACT Serology remains critical for diagnosing hepatitis D virus (HDV) infection, which affects 15 to 20 million people worldwide, but the literature on characterizing commercial enzyme immunoassays (EIAs) dates back to 15 years ago. We evaluated 2 commercial EIAs currently available for detecting anti-HDV antibodies. The DiaSorin assay demonstrated 100% sensitivity and specificity. Using a modified cutoff value, the Cusabio assay demonstrated a sensitivity of 81.3% and specificity of 90.9%. Our data show that recently developed EIAs are reliable for anti-HDV antibody detection.