Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Comparison of Enzyme Immunoassays for Detection of Antibodies to Hepatitis D Virus in Serum

Comparison of Enzyme Immunoassays for Detection of Antibodies to Hepatitis D Virus in Serum Comparison of Enzyme Immunoassays for Detection of Antibodies to Hepatitis D Virus in Serum Siu-Kei Chow a * , Ederlyn E. Atienza a , Linda Cook a , Harry Prince b * , Patricia Slev c , Mary Lapé-Nixon b and Keith R. Jerome a , d a Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA b Focus Diagnostics Reference Laboratory, San Juan Capistrano, California, USA c Department of Pathology, University of Utah, Salt Lake City, Utah, USA d The Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA R. L. Hodinka , Editor University of South Carolina School of Medicine Greenville ABSTRACT Serology remains critical for diagnosing hepatitis D virus (HDV) infection, which affects 15 to 20 million people worldwide, but the literature on characterizing commercial enzyme immunoassays (EIAs) dates back to 15 years ago. We evaluated 2 commercial EIAs currently available for detecting anti-HDV antibodies. The DiaSorin assay demonstrated 100% sensitivity and specificity. Using a modified cutoff value, the Cusabio assay demonstrated a sensitivity of 81.3% and specificity of 90.9%. Our data show that recently developed EIAs are reliable for anti-HDV antibody detection. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical and Vaccine Immunology American Society For Microbiology

Comparison of Enzyme Immunoassays for Detection of Antibodies to Hepatitis D Virus in Serum

Comparison of Enzyme Immunoassays for Detection of Antibodies to Hepatitis D Virus in Serum

Clinical and Vaccine Immunology , Volume 23 (8): 732 – Aug 1, 2016

Abstract

Comparison of Enzyme Immunoassays for Detection of Antibodies to Hepatitis D Virus in Serum Siu-Kei Chow a * , Ederlyn E. Atienza a , Linda Cook a , Harry Prince b * , Patricia Slev c , Mary Lapé-Nixon b and Keith R. Jerome a , d a Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA b Focus Diagnostics Reference Laboratory, San Juan Capistrano, California, USA c Department of Pathology, University of Utah, Salt Lake City, Utah, USA d The Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA R. L. Hodinka , Editor University of South Carolina School of Medicine Greenville ABSTRACT Serology remains critical for diagnosing hepatitis D virus (HDV) infection, which affects 15 to 20 million people worldwide, but the literature on characterizing commercial enzyme immunoassays (EIAs) dates back to 15 years ago. We evaluated 2 commercial EIAs currently available for detecting anti-HDV antibodies. The DiaSorin assay demonstrated 100% sensitivity and specificity. Using a modified cutoff value, the Cusabio assay demonstrated a sensitivity of 81.3% and specificity of 90.9%. Our data show that recently developed EIAs are reliable for anti-HDV antibody detection.

Loading next page...
 
/lp/american-society-for-microbiology/comparison-of-enzyme-immunoassays-for-detection-of-antibodies-to-3gwOSzGJSw

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
American Society For Microbiology
Copyright
Copyright © 2016 by the American society for Microbiology.
ISSN
1556-6811
eISSN
1556-679X
DOI
10.1128/CVI.00028-16
pmid
27280621
Publisher site
See Article on Publisher Site

Abstract

Comparison of Enzyme Immunoassays for Detection of Antibodies to Hepatitis D Virus in Serum Siu-Kei Chow a * , Ederlyn E. Atienza a , Linda Cook a , Harry Prince b * , Patricia Slev c , Mary Lapé-Nixon b and Keith R. Jerome a , d a Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA b Focus Diagnostics Reference Laboratory, San Juan Capistrano, California, USA c Department of Pathology, University of Utah, Salt Lake City, Utah, USA d The Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA R. L. Hodinka , Editor University of South Carolina School of Medicine Greenville ABSTRACT Serology remains critical for diagnosing hepatitis D virus (HDV) infection, which affects 15 to 20 million people worldwide, but the literature on characterizing commercial enzyme immunoassays (EIAs) dates back to 15 years ago. We evaluated 2 commercial EIAs currently available for detecting anti-HDV antibodies. The DiaSorin assay demonstrated 100% sensitivity and specificity. Using a modified cutoff value, the Cusabio assay demonstrated a sensitivity of 81.3% and specificity of 90.9%. Our data show that recently developed EIAs are reliable for anti-HDV antibody detection.

Journal

Clinical and Vaccine ImmunologyAmerican Society For Microbiology

Published: Aug 1, 2016

There are no references for this article.