A Novel Enzyme-Linked Immunosorbent Assay Using the Recombinant Actinobacillus pleuropneumoniae ApxII Antigen for Diagnosis of Pleuropneumonia in Pig Herds
Abstract
For the surveillance of pig herds infected with porcine pleuropneumonia, an enzyme-linked immunosorbent assay (ELISA) using the recombinant Actinobacillus pleuropneumoniae ApxII protein as species- but not serotype-specific antigen was developed. Using this ELISA, 243 of 400 animals from 22 A. pleuropneumoniae -infected herds were classified as seropositive.