Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Studies in Sickle-Cell Anemia: XII. Further Studies on Hepatic Function in Sickle-Cell Anemia

Studies in Sickle-Cell Anemia: XII. Further Studies on Hepatic Function in Sickle-Cell Anemia Abstract There is considerable clinical evidence of hepatic involvement in sickle-cell anemia (SCA). Clinically, the liver is enlarged and at times tender in 40% of young children with SCA, and jaundice is present in about 50% of cases after 5 years of age.1 The total circulating plasma protein mass is increased in these patients.2 This places an extra strain on the liver as a source of production of serum albumin. Despite clinical evidence of liver involvement in children with SCA, difficulty is usually encountered in quantitating hepatic dysfunction by the usual laboratory tests. This paper represents an attempt to obtain additional evidence of disturbance in liver metabolism in SCA. Methods The following tests were done: (a) traditional liver-function tests, (b) serum protein electrophoresis, (c) determination of the level of venous blood ammonia, (d) analysis of amino acid excretion in the urine, and (e) histological examination of liver tissue.A. References 1. Scott, R. B.; Ferguson, A. D.; Jenkins, M. E., and Clark, H. M.: Studies in Sickle-Cell Anemia: VIII. Further Observations on the Clinical Manifestations of Sickle-Cell Anemia in Children , A. M. A. Am. J. Dis. Child. 90:682, 1955. 2. Jenkins, M. E.; Scott, R. B., and Ferguson, A. D.: Studies in Sickle Cell Anemia: VII. Blood Volume Relationships and the Use of a Plasma Extender in Sickle Cell Disease in Childhood: A Preliminary Report , Pediatrics 18:239, 1956. 3. Fister, H. J.: Manual of Standardized Procedures for Spectrophotometric Chemistry, Standard Scientific Supply Corp., N. Y. 4. Powell, W. N.: Total Bilirubin in Serum , Am. J. Clin. Path., Tech. Sect. 14:56, 1944. 5. Hsia, D. Y.; Hsia, H. H., and Gellis, S. S.: A Micromethod for Serum Bilirubin , J. Lab. & Clin. Med. 40:610, 1952. 6. Hanger, F. M.: Serological Differentiation of Obstructive from Hepatogenous Jaundice by Flocculation of Cephalin-Cholesterol Emulsions , J. Clin. Invest. 18:261, 1939. 7. Karmen, A.; Wroblewski, F., and LaDue, J. S.: Transaminase Activity in Human Blood , J. Clin. Invest. 34:126, 1955. 8. McDermott, M. V., Jr.; Adams, R. D., and Riddell, A. G.: Ammonia Metabolism in Man , Ann. Surg. 140:539, 1954. 9. Schwartz, R.; Phillips, G. B.; Gabuzda, G. J., Jr., and Davidson, C. S.: Blood Ammonia and Electrolytes in Hepatic Coma , J. Lab. & Clin. Med. 42:499, 1953. 10. Walshe, J. M.: Observations on the Symptomatology and Pathogenesis of Hepatic Coma , Quart. J. Med. 20:421, 1951. 11. Seligson, D., and Seligson, H.: A Microdiffusion Method for the Determination of Nitrogen Liberated as Ammonia , J. Lab. & Clin. Med. 38: 324, 1951. 12. Dent, C. E.: Chromatography in the Studies of Amino Acid Metabolism , in Lectures on the Scientific Basis of Medicine , London, Athlone Press, 1953, Vol. 2, p. 213. 13. Evered, D. F.: The Excretion of Amino Acids by the Human , Biochem. J. 62:416, 1956. 14. Fenichel, R. L.; Watson, J., and Eirich, F.: Electrophoretic Studies of the Plasma and Serum Proteins in Sickle Cell Anemia , J. Clin. Invest. 29:1620, 1950. 15. Stowens, D.: Personal communication to the authors. 16. Bruton, O. C.; Metzger, J. F., and Sprinz, H.: Experience with Needle Biopsy of Liver in Infants and Children , Pediatrics 16:836, 1955. 17. Green, T. W.; Conley, C. L., and Berthrong, M.: The Liver in Sickle Cell Anemia , Bull. Johns Hopkins Hosp. 92:99, 1953. 18. Song, Y. S.: Hepatic Lesions in Sickle Cell Anemia , Am. J. Path. 33:331, 1957. 19. Wolman, I. J.: Laboratory Applications in Clinical Pediatrics , New York, Blakiston Company (division of McGraw-Hill Book Company, Inc.), 1957. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png A.M.A. Journal of Diseases of Children American Medical Association

Studies in Sickle-Cell Anemia: XII. Further Studies on Hepatic Function in Sickle-Cell Anemia

Loading next page...
 
/lp/american-medical-association/studies-in-sickle-cell-anemia-xii-further-studies-on-hepatic-function-j3HJCpJutx

References (20)

Publisher
American Medical Association
Copyright
Copyright © 1959 American Medical Association. All Rights Reserved.
ISSN
0096-6916
DOI
10.1001/archpedi.1959.02070010420006
Publisher site
See Article on Publisher Site

Abstract

Abstract There is considerable clinical evidence of hepatic involvement in sickle-cell anemia (SCA). Clinically, the liver is enlarged and at times tender in 40% of young children with SCA, and jaundice is present in about 50% of cases after 5 years of age.1 The total circulating plasma protein mass is increased in these patients.2 This places an extra strain on the liver as a source of production of serum albumin. Despite clinical evidence of liver involvement in children with SCA, difficulty is usually encountered in quantitating hepatic dysfunction by the usual laboratory tests. This paper represents an attempt to obtain additional evidence of disturbance in liver metabolism in SCA. Methods The following tests were done: (a) traditional liver-function tests, (b) serum protein electrophoresis, (c) determination of the level of venous blood ammonia, (d) analysis of amino acid excretion in the urine, and (e) histological examination of liver tissue.A. References 1. Scott, R. B.; Ferguson, A. D.; Jenkins, M. E., and Clark, H. M.: Studies in Sickle-Cell Anemia: VIII. Further Observations on the Clinical Manifestations of Sickle-Cell Anemia in Children , A. M. A. Am. J. Dis. Child. 90:682, 1955. 2. Jenkins, M. E.; Scott, R. B., and Ferguson, A. D.: Studies in Sickle Cell Anemia: VII. Blood Volume Relationships and the Use of a Plasma Extender in Sickle Cell Disease in Childhood: A Preliminary Report , Pediatrics 18:239, 1956. 3. Fister, H. J.: Manual of Standardized Procedures for Spectrophotometric Chemistry, Standard Scientific Supply Corp., N. Y. 4. Powell, W. N.: Total Bilirubin in Serum , Am. J. Clin. Path., Tech. Sect. 14:56, 1944. 5. Hsia, D. Y.; Hsia, H. H., and Gellis, S. S.: A Micromethod for Serum Bilirubin , J. Lab. & Clin. Med. 40:610, 1952. 6. Hanger, F. M.: Serological Differentiation of Obstructive from Hepatogenous Jaundice by Flocculation of Cephalin-Cholesterol Emulsions , J. Clin. Invest. 18:261, 1939. 7. Karmen, A.; Wroblewski, F., and LaDue, J. S.: Transaminase Activity in Human Blood , J. Clin. Invest. 34:126, 1955. 8. McDermott, M. V., Jr.; Adams, R. D., and Riddell, A. G.: Ammonia Metabolism in Man , Ann. Surg. 140:539, 1954. 9. Schwartz, R.; Phillips, G. B.; Gabuzda, G. J., Jr., and Davidson, C. S.: Blood Ammonia and Electrolytes in Hepatic Coma , J. Lab. & Clin. Med. 42:499, 1953. 10. Walshe, J. M.: Observations on the Symptomatology and Pathogenesis of Hepatic Coma , Quart. J. Med. 20:421, 1951. 11. Seligson, D., and Seligson, H.: A Microdiffusion Method for the Determination of Nitrogen Liberated as Ammonia , J. Lab. & Clin. Med. 38: 324, 1951. 12. Dent, C. E.: Chromatography in the Studies of Amino Acid Metabolism , in Lectures on the Scientific Basis of Medicine , London, Athlone Press, 1953, Vol. 2, p. 213. 13. Evered, D. F.: The Excretion of Amino Acids by the Human , Biochem. J. 62:416, 1956. 14. Fenichel, R. L.; Watson, J., and Eirich, F.: Electrophoretic Studies of the Plasma and Serum Proteins in Sickle Cell Anemia , J. Clin. Invest. 29:1620, 1950. 15. Stowens, D.: Personal communication to the authors. 16. Bruton, O. C.; Metzger, J. F., and Sprinz, H.: Experience with Needle Biopsy of Liver in Infants and Children , Pediatrics 16:836, 1955. 17. Green, T. W.; Conley, C. L., and Berthrong, M.: The Liver in Sickle Cell Anemia , Bull. Johns Hopkins Hosp. 92:99, 1953. 18. Song, Y. S.: Hepatic Lesions in Sickle Cell Anemia , Am. J. Path. 33:331, 1957. 19. Wolman, I. J.: Laboratory Applications in Clinical Pediatrics , New York, Blakiston Company (division of McGraw-Hill Book Company, Inc.), 1957.

Journal

A.M.A. Journal of Diseases of ChildrenAmerican Medical Association

Published: Apr 1, 1959

There are no references for this article.