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A Rapid Micromethod for the Determination of Indirect Bilirubin

A Rapid Micromethod for the Determination of Indirect Bilirubin Abstract Since the indictment of bilirubin as an etiologic factor in the development of kern-icterus, widespread attention has been focused upon neonatal jaundice. Frequent bilirubin determinations are now being done on small infants, and a micromethod that obviates the necessity for venipunctures and at the same time can be easily performed would seem desirable. Because the bilirubin producing icterus in the first week of life, associated with erythroblastosis, sepsis, or prematurity, is almost entirely indirect-reacting, a simple method for determination of indirect bilirubin alone should be of practical value. Such a method, based on that originally described by Ernst and Förster1 and modified by Weech and co-workers * in 1941, is now in use at the Children's Hospital. The addition of acetone to serum precipitates direct bilirubin together with protein and leaves indirect bilirubin in the clear yellow supernate, where its concentration can be determined photometrically. As described by Davidson, Merritt, References 1. Ernst, Z., and Förster, J.: Colorimetric Test for Bilirubin in Blood , Klin. Wchnschr. 3: 2386 ( (Dec.) ) 1924.Crossref 2. Weech, A. A.; Vann, D., and Grillo, R. A.: Clearance of Bilirubin from the Plasma: Measure of the Excreting Power of the Liver , J. Clin. Invest. 20:323 ( (May) ) 1941.Crossref 3. Davidson, L. T.; Merritt, K. K., and Weech, A. A.: Hyperbilirubinemia in the Newborn , Am. J. Child. 61:958 ( (May) ) 1941. 4. Malloy, H. T., and Evelyn, K. A.: Determiation of Bilirubin with the Photoelectric Colorimeter , J. Biol. Chem. 119:481 ( (July) ) 1937. 5. Najjar, V. A., and Childs, B.: Crystallization and Properties of Serum Bilirubin , J. Biol. Chem. 204:359 ( (Sept.) ) 1953. 6. Stoner, W. C.: Carotinemia , Am. J. M. Sc. 175:31 ( (Jan.) ) 1928.Crossref 7. Hsia, D. Y. Y.; Hsia, H. H., and Gellis, S. S.: Micromethod for Serum Bilirubin , J. Lab. & Clin. Med. 40:610 ( (Oct.) ) 1952. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png A.M.A. Journal of Diseases of Children American Medical Association

A Rapid Micromethod for the Determination of Indirect Bilirubin

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References (8)

Publisher
American Medical Association
Copyright
Copyright © 1956 American Medical Association. All Rights Reserved.
ISSN
0096-6916
DOI
10.1001/archpedi.1956.02060020021004
Publisher site
See Article on Publisher Site

Abstract

Abstract Since the indictment of bilirubin as an etiologic factor in the development of kern-icterus, widespread attention has been focused upon neonatal jaundice. Frequent bilirubin determinations are now being done on small infants, and a micromethod that obviates the necessity for venipunctures and at the same time can be easily performed would seem desirable. Because the bilirubin producing icterus in the first week of life, associated with erythroblastosis, sepsis, or prematurity, is almost entirely indirect-reacting, a simple method for determination of indirect bilirubin alone should be of practical value. Such a method, based on that originally described by Ernst and Förster1 and modified by Weech and co-workers * in 1941, is now in use at the Children's Hospital. The addition of acetone to serum precipitates direct bilirubin together with protein and leaves indirect bilirubin in the clear yellow supernate, where its concentration can be determined photometrically. As described by Davidson, Merritt, References 1. Ernst, Z., and Förster, J.: Colorimetric Test for Bilirubin in Blood , Klin. Wchnschr. 3: 2386 ( (Dec.) ) 1924.Crossref 2. Weech, A. A.; Vann, D., and Grillo, R. A.: Clearance of Bilirubin from the Plasma: Measure of the Excreting Power of the Liver , J. Clin. Invest. 20:323 ( (May) ) 1941.Crossref 3. Davidson, L. T.; Merritt, K. K., and Weech, A. A.: Hyperbilirubinemia in the Newborn , Am. J. Child. 61:958 ( (May) ) 1941. 4. Malloy, H. T., and Evelyn, K. A.: Determiation of Bilirubin with the Photoelectric Colorimeter , J. Biol. Chem. 119:481 ( (July) ) 1937. 5. Najjar, V. A., and Childs, B.: Crystallization and Properties of Serum Bilirubin , J. Biol. Chem. 204:359 ( (Sept.) ) 1953. 6. Stoner, W. C.: Carotinemia , Am. J. M. Sc. 175:31 ( (Jan.) ) 1928.Crossref 7. Hsia, D. Y. Y.; Hsia, H. H., and Gellis, S. S.: Micromethod for Serum Bilirubin , J. Lab. & Clin. Med. 40:610 ( (Oct.) ) 1952.

Journal

A.M.A. Journal of Diseases of ChildrenAmerican Medical Association

Published: Jan 1, 1956

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