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Schistosoma japonicum: Protective Immunity Induced by Schistosomulum-Derived Cells in a Mouse Model

Schistosoma japonicum: Protective Immunity Induced by Schistosomulum-Derived Cells in a Mouse Model We previously reported that immunization with intact live cells from schistosomula of Schistosoma japonicum (S.j) partially protected the Kunming strain of mice from challenge infection. In the present work, 2 immune protective experiments were designed to further validate the protective effect induced by this type of vaccine and to optimize the immunization protocol, including the number of inoculations and parasite stages from which immunogenic cells were derived. Three antigens derived from 18-day-old postinfection live (LLC) and dead (DLC) larval worm cells and from dead 42-day-old postinfection adult worm cells (DAC) were used as immunogens. Our results demonstrate that live cells from 18-day-old worms are capable of inducing significant protection in mice using a murine-Sj challenge model as shown by reduction rates of worm recoveries and egg burdens. The development of adult worms was stunted. A Th1-biased immune response was reflected in the protected groups as evidenced by the ratio of IgG2a/IgG1. A 38-kDa polypeptide was recognized by sera from LLC immunized animals. We demonstrate that live parasite cells are a source of novel protective antigens that can be exploited for vaccine development. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Parasitology Allen Press

Schistosoma japonicum: Protective Immunity Induced by Schistosomulum-Derived Cells in a Mouse Model

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The Journal of Parasitology , Volume 94 (2): 9 – Apr 28, 2008

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References (54)

Publisher
Allen Press
Copyright
American Society of Parasitologists
Subject
Immunology
ISSN
0022-3395
eISSN
1937-2345
DOI
10.1645/GE-1315.1
pmid
18564740
Publisher site
See Article on Publisher Site

Abstract

We previously reported that immunization with intact live cells from schistosomula of Schistosoma japonicum (S.j) partially protected the Kunming strain of mice from challenge infection. In the present work, 2 immune protective experiments were designed to further validate the protective effect induced by this type of vaccine and to optimize the immunization protocol, including the number of inoculations and parasite stages from which immunogenic cells were derived. Three antigens derived from 18-day-old postinfection live (LLC) and dead (DLC) larval worm cells and from dead 42-day-old postinfection adult worm cells (DAC) were used as immunogens. Our results demonstrate that live cells from 18-day-old worms are capable of inducing significant protection in mice using a murine-Sj challenge model as shown by reduction rates of worm recoveries and egg burdens. The development of adult worms was stunted. A Th1-biased immune response was reflected in the protected groups as evidenced by the ratio of IgG2a/IgG1. A 38-kDa polypeptide was recognized by sera from LLC immunized animals. We demonstrate that live parasite cells are a source of novel protective antigens that can be exploited for vaccine development.

Journal

The Journal of ParasitologyAllen Press

Published: Apr 28, 2008

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