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INTERRELATIONSHIPS OF DICTYOCAULUS SPP. IN WILD RUMINANTS WITH MORPHOLOGICAL DESCRIPTION OF DICTYOCAULUS CERVI N. SP. (NEMATODA: TRICHOSTRONGYLOIDEA) FROM RED DEER, CERVUS ELAPHUS

INTERRELATIONSHIPS OF DICTYOCAULUS SPP. IN WILD RUMINANTS WITH MORPHOLOGICAL DESCRIPTION OF... AbstractLungworms from the genus Dictyocaulus cause parasitic bronchitis (dictyocaulosis) characterized by coughing and severe lung pathology in both domestic and wild ruminants. In this study we investigated the interrelationships of Dictyocaulus spp. from European bison (Bison bonasus L.), roe deer (Capreolus capreolus) and red deer (Cervus elaphus) by nucleotide sequence analysis spanning the 18S RNA gene (SSU) and internal transcribed spacer 2 (ITS2) regions of the ribosomal gene array as well as the mitochondrial cytochrome c oxidase subunit 1 (cox1). Molecular analyses of sequence data obtained partly with novel primers, from between 10 and 50 specimens from each host were carried out. Bayesian inference (BI) analysis revealed that each host species was infected with different genotypes. Analysis of cox1 sequence data showed a diverse genetic background and high evolutionary potential of Dictyocaulus taxa. Data from lungworms of European bison revealed a distinct genotype of D. viviparus, whereas D. capreolus was only found in roe deer. In contrast, red deer were infected with a taxon with unique SSU, ITS2 and cox1 sequences. These results indicate the occurrence of a novel genotype from red deer, which differs significantly from the NCBI reference sequence of D. eckerti. The molecular evidence was consistent with a morphological study with description and imaging of Dictyocaulus cervi n. sp. recovered from red deer. Dictyocaulus cervi n. sp. can be distinguished from Dictyocaulus eckerti on the basis of the absence of cervical papillae, the occurrence of a single ring of 4 symmetrical submedian cephalic papillae, length of the tail in females, morphometry of the female reproductive system and measurements of gubernacula in males. In conclusion, our findings further strengthen the idea that the genetic complexity and diversity among Dictyocaulus lungworms infecting wildlife ruminants is larger than previously believed and warrants further investigation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Parasitology Allen Press

INTERRELATIONSHIPS OF DICTYOCAULUS SPP. IN WILD RUMINANTS WITH MORPHOLOGICAL DESCRIPTION OF DICTYOCAULUS CERVI N. SP. (NEMATODA: TRICHOSTRONGYLOIDEA) FROM RED DEER, CERVUS ELAPHUS

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The Journal of Parasitology : 1 – Jun 16, 2016

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References (33)

Publisher
Allen Press
Copyright
American Society of Parasitologists 2017
Subject
Regular Article
ISSN
0022-3395
eISSN
1937-2345
DOI
10.1645/16-75
pmid
28585897
Publisher site
See Article on Publisher Site

Abstract

AbstractLungworms from the genus Dictyocaulus cause parasitic bronchitis (dictyocaulosis) characterized by coughing and severe lung pathology in both domestic and wild ruminants. In this study we investigated the interrelationships of Dictyocaulus spp. from European bison (Bison bonasus L.), roe deer (Capreolus capreolus) and red deer (Cervus elaphus) by nucleotide sequence analysis spanning the 18S RNA gene (SSU) and internal transcribed spacer 2 (ITS2) regions of the ribosomal gene array as well as the mitochondrial cytochrome c oxidase subunit 1 (cox1). Molecular analyses of sequence data obtained partly with novel primers, from between 10 and 50 specimens from each host were carried out. Bayesian inference (BI) analysis revealed that each host species was infected with different genotypes. Analysis of cox1 sequence data showed a diverse genetic background and high evolutionary potential of Dictyocaulus taxa. Data from lungworms of European bison revealed a distinct genotype of D. viviparus, whereas D. capreolus was only found in roe deer. In contrast, red deer were infected with a taxon with unique SSU, ITS2 and cox1 sequences. These results indicate the occurrence of a novel genotype from red deer, which differs significantly from the NCBI reference sequence of D. eckerti. The molecular evidence was consistent with a morphological study with description and imaging of Dictyocaulus cervi n. sp. recovered from red deer. Dictyocaulus cervi n. sp. can be distinguished from Dictyocaulus eckerti on the basis of the absence of cervical papillae, the occurrence of a single ring of 4 symmetrical submedian cephalic papillae, length of the tail in females, morphometry of the female reproductive system and measurements of gubernacula in males. In conclusion, our findings further strengthen the idea that the genetic complexity and diversity among Dictyocaulus lungworms infecting wildlife ruminants is larger than previously believed and warrants further investigation.

Journal

The Journal of ParasitologyAllen Press

Published: Jun 16, 2016

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