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Human Malaria Parasites in Continuous Culture

Human Malaria Parasites in Continuous Culture Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38°C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen (1 or 5 percent). The original parasite material, derived from an infected Aotus trivirgatus monkey, was diluted more than 100 million times by the addition of human erythrocytes at 3- or 4-day intervals. The parasites continued to reproduce in their normal asexual cycle of approximately 48 hours but were no longer highly synchronous. They have remained infective to Aotus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Parasitology Allen Press

Human Malaria Parasites in Continuous Culture

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The Journal of Parasitology , Volume 91 (3): 3 – Jun 1, 2005

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Publisher
Allen Press
Copyright
American Society of Parasitologists
Subject
DEFINING THE FIELD
ISSN
0022-3395
eISSN
1937-2345
DOI
10.1645/0022-3395(2005)091[0484:HMPICC]2.0.CO;2
Publisher site
See Article on Publisher Site

Abstract

Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38°C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen (1 or 5 percent). The original parasite material, derived from an infected Aotus trivirgatus monkey, was diluted more than 100 million times by the addition of human erythrocytes at 3- or 4-day intervals. The parasites continued to reproduce in their normal asexual cycle of approximately 48 hours but were no longer highly synchronous. They have remained infective to Aotus.

Journal

The Journal of ParasitologyAllen Press

Published: Jun 1, 2005

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