Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/allen-press/development-of-a-droplet-digital-pcr-for-detection-of-trichuriasis-in-e8LoA7f9l0
References (39)
-
Yan Wang, Guo-Hua Liu, Jia-Yuan Li, Min-jun Xu, Yong-gang Ye, Dong‐Hui Zhou, Hui Song, R. Lin, Xing-Quan Zhu (2013)
Genetic variability among Trichuris ovis isolates from different hosts in Guangdong Province, China revealed by sequences of three mitochondrial genesMitochondrial DNA, 24
-
J. Demeler, Sabrina Ramünke, S. Wolken, Davide Ianiello, L. Rinaldi, J. Gahutu, G. Cringoli, G. Samson-Himmelstjerna, J. Krücken (2013)
Discrimination of Gastrointestinal Nematode Eggs from Crude Fecal Egg Preparations by Inhibitor-Resistant Conventional and Real-Time PCRPLoS ONE, 8
-
R. Eichenberger, M. Talukder, M. Field, P. Wangchuk, P. Giacomin, A. Loukas, J. Sotillo (2018)
Characterization of Trichuris muris secreted proteins and extracellular vesicles provides new insights into host–parasite communicationJournal of Extracellular Vesicles, 7
-
K. Weerakoon, C. Gordon, Gail Williams, Pengfei Cai, G. Gobert, R. Olveda, A. Ross, David Olveda, D. McManus (2017)
Droplet Digital PCR Diagnosis of Human Schistosomiasis: Parasite Cell-Free DNA Detection in Diverse Clinical SamplesThe Journal of Infectious Diseases, 216
-
Guo-Hua Liu, Yan Wang, Min-jun Xu, Dong‐Hui Zhou, Yong-gang Ye, Jia-Yuan Li, Hui Song, R. Lin, Xing-Quan Zhu (2012)
Characterization of the complete mitochondrial genomes of two whipworms Trichuris ovis and Trichuris discolor (Nematoda: Trichuridae).Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 12 8
-
C. Cutillas, P. German, P. Arias, D. Guevara (1995)
Trichuris ovis and Trichuris globulosa: morphological, biometrical, and genetic studies.Experimental parasitology, 81 4
-
Rojelio Mejia, Yosselin Vicuña, Nely Broncano, C. Sandoval, M. Vaca, M. Chico, P. Cooper, T. Nutman (2013)
A novel, multi-parallel, real-time polymerase chain reaction approach for eight gastrointestinal parasites provides improved diagnostic capabilities to resource-limited at-risk populations.The American journal of tropical medicine and hygiene, 88 6
-
Florian Roeber, L. Kahn (2014)
The specific diagnosis of gastrointestinal nematode infections in livestock: larval culture technique, its limitations and alternative DNA-based approaches.Veterinary parasitology, 205 3-4
-
Y. Sanke (2013)
Establishment of specific PCR for the detection of Trichuris globulosa in goatsChinese Veterinary Science
-
N. Mohd-Shaharuddin, Y. Lim, N. Hassan, S. Nathan, R. Ngui (2019)
Molecular characterization of Trichuris species isolated from humans, dogs and cats in a rural community in Peninsular Malaysia.Acta tropica, 190
-
Ruth Sedlak, K. Jerome (2014)
The potential advantages of digital PCR for clinical virology diagnosticsExpert Review of Molecular Diagnostics, 14
-
C. Cutillas, R. Oliveros, M. Rojas, D. Guevara (2004)
Determination of Trichuris skrjabini by Sequencing of the ITS1–5.8S–ITS2 Segment of the Ribosomal DNA: Comparative Molecular Study of Different Species of Trichurids, 90
-
Sofie Nissen, A. Al-Jubury, Tina Hansen, A. Olsen, H. Christensen, S. Thamsborg, P. Nejsum (2012)
Genetic analysis of Trichuris suis and Trichuris trichiura recovered from humans and pigs in a sympatric setting in Uganda.Veterinary parasitology, 188 1-2
-
P. Baltrušis, P. Halvarsson, J. Höglund (2019)
Molecular detection of two major gastrointestinal parasite genera in cattle using a novel droplet digital PCR approachParasitology Research, 118
-
Maria Kaisar, E. Brienen, Y. Djuardi, E. Sartono, M. Yazdanbakhsh, J. Verweij, T. Supali, L. Lieshout (2017)
Improved diagnosis of Trichuris trichiura by using a bead-beating procedure on ethanol preserved stool samples prior to DNA isolation and the performance of multiplex real-time PCR for intestinal parasitesParasitology, 144
-
Zhixun Xie, S. Luo, Liji Xie, Jia-bo Liu, Y. Pang, Xianwen Deng, Zhiqin Xie, Qing Fan, Mazhar Khan (2014)
Simultaneous typing of nine avian respiratory pathogens using a novel GeXP analyzer-based multiplex PCR assay.Journal of virological methods, 207
-
I. Phosuk, O. Sanpool, T. Thanchomnang, L. Sadaow, Rutchanee Rodpai, Witthaya Anamnart, P. Janwan, A. Wijit, S. Laymanivong, Win Aung, P. Intapan, W. Maleewong (2018)
Molecular Identification of Trichuris suis and Trichuris trichiura Eggs in Human Populations from Thailand, Lao PDR, and Myanmar.The American journal of tropical medicine and hygiene, 98 1
-
Meishen Ren, Hua Lin, Shi-Jie Chen, Miao Yang, Wei An, Yin Wang, C. Xue, Yinjie Sun, Yubao Yan, Juan Hu (2018)
Detection of pseudorabies virus by duplex droplet digital PCR assayJournal of Veterinary Diagnostic Investigation, 30
-
R. Pullan, Jennifer Smith, Rashmi Jasrasaria, S. Brooker (2014)
Global numbers of infection and disease burden of soil transmitted helminth infections in 2010Parasites & Vectors, 7
-
A. Ahmad, M. Shabbir, Y. Xin, Muhammad Ikram, M. Hafeez, Chunqun Wang, Ting Zhang, Caixian Zhou, Xingrun Yan, Mubashar Hassan, Min Hu (2019)
Characterization of the Complete Mitochondrial Genome of a Whipworm Trichuris skrjabini (Nematoda: Trichuridae)Genes, 10
-
R. Cimino, Rebecca Jeun, M. Juárez, P. Cajal, Paola Vargas, Adriana Echazú, Patricia Bryan, J. Nasser, A. Krolewiecki, Rojelio Mejia (2015)
Identification of human intestinal parasites affecting an asymptomatic peri-urban Argentinian population using multi-parallel quantitative real-time polymerase chain reactionParasites & Vectors, 8
-
Yongning Zhang, Zhou Zhang, Zhan-Wei Wang, Zili Wang, Caixia Wang, Chunyan Feng, W. Yuan, Xiangmei Lin, Shaoqiang Wu (2019)
Development of a droplet digital PCR assay for sensitive detection of porcine circovirus 3.Molecular and cellular probes, 43
-
J. Bethony, S. Brooker, M. Albonico, S. Geiger, A. Loukas, D. Diemert, P. Hotez (2006)
Soil-transmitted helminth infections: ascariasis, trichuriasis, and hookwormThe Lancet, 367
-
Janina Ferrand, K. Patron, C. Legrand-Frossi, J. Frippiat, C. Merlin, C. Alauzet, A. Lozniewski (2014)
Comparison of seven methods for extraction of bacterial DNA from fecal and cecal samples of mice.Journal of microbiological methods, 105
-
G. Liu, W. Zhou, A. Nisbet, M. Xu, D.H. Zhou, G. Zhao, S. Wang, H.Q. Song, R. Lin, X. Zhu (2012)
Characterization of Trichuris trichiura from humans and T. suis from pigs in China using internal transcribed spacers of nuclear ribosomal DNAJournal of Helminthology, 88
-
P. Baltrušis, P. Halvarsson, J. Höglund (2018)
Exploring benzimidazole resistance in Haemonchus contortus by next generation sequencing and droplet digital PCRInternational Journal for Parasitology: Drugs and Drug Resistance, 8
-
Yiping Cao, Meredith Raith, J. Griffith (2015)
Droplet digital PCR for simultaneous quantification of general and human-associated fecal indicators for water quality assessment.Water research, 70
-
S. Car, C. Croton, M. Haworth (2019)
Pseudohypoadrenocorticism in a Siberian Husky with Trichuris vulpis InfectionCase Reports in Veterinary Medicine, 2019
-
E. Pomari, C. Piubelli, F. Perandin, Z. Bisoffi (2019)
Digital PCR: a new technology for diagnosis of parasitic infections.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
-
Safaa Elmahalawy, P. Halvarsson, Moa Skarin, J. Höglund (2018)
Droplet digital polymerase chain reaction (ddPCR) as a novel method for absolute quantification of major gastrointestinal nematodes in sheep.Veterinary parasitology, 261
-
T. Singh, S. Lal (2011)
A study on in vitro culture of Trichuris ovis in different physiological solutions at constant temperature, 37°CJournal of Parasitic Diseases, 35
-
Jessica Grant, N. Pilotte, Steven Williams (2019)
A Case for Using Genomics and a Bioinformatics Pipeline to Develop Sensitive and Species-Specific PCR-Based Diagnostics for Soil-Transmitted HelminthsFrontiers in Genetics, 10
-
I. Amoah, Gulshan Singh, T. Stenström, P. Reddy (2017)
Detection and quantification of soil-transmitted helminths in environmental samples: A review of current state-of-the-art and future perspectives.Acta tropica, 169
-
P. Iliev, A. Ivanov, Z. Kirkova, K. Hristov, K. Dinkova, J. Ananiev (2017)
Some parasitological, pathological and immunohistochemical examinations in sheep naturally infected by Trichuris ovisThe Journal of Supercomputing, 15
-
L. Tan, Aibing Wang, J. Yi, Yisong Liu, Jiayu Li, Wei Liu (2018)
Prevalence and Phylogenetic Analyses of Trichuris suis in Pigs in Hunan Province, Subtropical ChinaThe Korean Journal of Parasitology, 56
-
I. Gutiérrez-Aguirre, N. Rački, T. Dreo, M. Ravnikar (2015)
Droplet digital PCR for absolute quantification of pathogens.Methods in molecular biology, 1302
-
V. Yevstafieva, I. Yuskiv, V. Melnychuk, I. Yasnolob, V. Kovalenko, K. Horb (2018)
Nematodes of the Genus Trichuris (Nematoda, Trichuridae), Parasitizing Sheep in Central and South-Eastern Regions of UkraineVestnik Zoologii, 52
-
Leonardo Pinheiro, V. Coleman, Christopher Hindson, J. Herrmann, B. Hindson, Somanath Bhat, K. Emslie (2011)
Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number QuantificationAnalytical Chemistry, 84
-
F. Partridge, Ruth Forman, N. Willis, C. Bataille, Emma Murphy, Anwen Brown, N. Heyer-Chauhan, Bruno Marinic, Daniel Sowood, G. Wynne, K. Else, A. Russell, D. Sattelle (2018)
2,4-Diaminothieno[3,2-d]pyrimidines, a new class of anthelmintic with activity against adult and egg stages of whipwormPLoS Neglected Tropical Diseases, 12
- Publisher
- Allen Press
- Copyright
- © American Society of Parasitologists 2020
- ISSN
- 0022-3395
- eISSN
- 1937-2345
- DOI
- 10.1645/20-16
- Publisher site
- See Article on Publisher Site
Abstract
ABSTRACTTrichuriasis is a serious threat to the economic development of animal husbandry. This research aimed to establish a droplet digital PCR (ddPCR) method to detect Trichuris spp. for the early diagnosis and prevention of trichuriasis in sheep. The real-time quantitative PCR (qPCR) and ddPCR methods were used for the detection of nematodes by targeted amplification of the ITS gene. Each means was evaluated to optimize the limit of detection and reproducibility. For a recombinant plasmid, the qPCR results showed that the detection limit was 31.7 copies per reaction. In contrast to qPCR, ddPCR was able to detect concentrations below 3.17 copies per reaction. Both assays exhibited good reproducibility. However, the ddPCR method was more stable for low-copy-number detection. This new assay was specific for Trichuris spp. and did not cross-react with other relevant gastrointestinal nematodes. A total of 98 clinical samples were tested with both assays. The results showed that the positive rate of ddPCR (80.6%) was higher than that of qPCR (72.4%). This method could be used as an efficient molecular biology tool to test for Trichuris spp. and could be a new valuable tool for the clinical diagnosis and prevention of trichuriasis.
Journal
The Journal of Parasitology – Allen Press
Published: Oct 30, 2020