Russian Journal of Bioorganic Chemistry Volume 26 Issue 1

Journal Article

Photoactivatable derivatives of peptide and protein ligands in the study of neuroreceptor structure

Utkin, Yu.

2007 Russian Journal of Bioorganic Chemistry

The application of photoactivatable derivatives for studies of different types of neuroreceptors belonging to two superfamilies, G-protein-dependent receptors and ligand-gated ionic channels, is discussed. Studies of the structure of voltage-gated and ion-gated channels with the use of specific...

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Hybridization properties and nuclease resistance of oligo(2′-O-tetrahydropyranylribonucleotides)

Kuznetsova, M.; Pyshnyi, D.; Repkova, M.; Venyaminova, A.

2007 Russian Journal of Bioorganic Chemistry

doi:10.1007/BF02758863

Oligo(2′-O-tetrahydropyranylribonucleotides) and their analogues containing a 3′-3′-internucleotide bond at the 3′-terminus are nuclease-resistant and possess rather high affinity toward RNA, the main target in the antisense approach.

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The isolation, preliminary structural studies, and physiological activity of water-soluble polysaccharides from the squeezed berries of the snowball treeViburnum opulus

Ovodova, R.; Golovchenko, V.; Popov, S.; Shashkov, A.; Ovodov, Yu.

2007 Russian Journal of Bioorganic Chemistry

doi:10.1007/BF02758861

Water-soluble polysaccharide fractions VO1–VO4 were isolated from the squeezed berries of the snowball tree (Viburnum opulus) by successive extraction with water at various temperatures and pH and with aqueous solutions of ammonium oxalate. These fractions were purified by ion-exchange...

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The inhibition of complement-dependent hemolysis by liposomes containing cerebroside sulfate

Kaplun, A.; Burdelev, O.; Ivanova, N.; Krasnopol’skii, Yu.; Shvets, V.

2007 Russian Journal of Bioorganic Chemistry

doi:10.1007/BF02758862

Cerebroside sulfate (CGS) was found to be capable of inhibiting complement-dependent hemolysis. The activity dependence of CGS-containing liposomes on their composition was studied. Mixtures of CGS with phosphatidylethanolamine, phosphatidylserine, sphingomyelin from cattle brain, cerebroside...

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Journal Article

Analytical biotechnology of recombinant peptides and proteins: I. determination of the purity, composition, and structure of human, porcine, and bovine insulins

Sergeev, N.; Nazimov, I.; Gavrikov, V.; Miroshnikov, A.

2007 Russian Journal of Bioorganic Chemistry

doi:10.1007/BF02758857

A method for analysis of the type, purity, and possible structural modifications of insulins of bovine, porcine, and human origin was proposed. It is based on a combination of narrow-bore reversed-phase HPLC and mass spectrometry. The hydrolysis of insulins with highly specific Glu-protease V8...

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The degradation of glycoproteins with lithium borohydride: Isolation and analysis ofO-glycopeptides with reducedC-terminal amino acid residue

Arbatsky, N.; Likhosherstov, L.; Serebryakova, M.; Brusov, O.; Shibaev, V.;

2007 Russian Journal of Bioorganic Chemistry

doi:10.1007/BF02758860

By the example of fetuin and a blood-group-specific mucin from porcine stomach, we showed that, under conditions of reductive degradation of glycoproteins with LiBH4-LiOH in 70% aqueoustert-butyl alcohol, the reduction and cleavage of amide bonds occur much faster than the simultaneous...

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Journal Article

Chemical reactions catalyzed by DNA polymerases

Krayevsky, A.

2007 Russian Journal of Bioorganic Chemistry

doi:10.1007/BF02758854

Chemical reactions catalyzed by various DNA polymerases are discussed, including DNA chain extension, the 3′→5′-exonuclease proofreading activity, and some other pathways of replicative repair. The contribution of DNA polymerases to the fidelity of the template-dependent synthesis is analyzed by...

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Journal Article

25th anniversary ofBioorganicheskaya Khimiya

Ivanov, V.T.; Berlin, Yu.

2007 Russian Journal of Bioorganic Chemistry

doi:10.1007/BF02758853

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Synthesis and fluorescent properties of 5-(1-pyrenylethynyl)-2′-deoxyuridine-containing oligodeoxynucleotides

Malakhov, A.; Malakhova, E.; Kuznitsova, S.; Grechishnikova, I.; Prokhorenko, I.;

2007 Russian Journal of Bioorganic Chemistry

doi:10.1007/BF02758859

Novel reagents for the fluorescent labeling of oligo- and polynucleotides have been prepared: 5-(1-pyrenylethynyl)-2′-deoxyuridine 3′-phosphoramidite and a solid support carrying this nucleoside. Oligo-nucleotides containing one or several modified units have been synthesized, and the...

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Russian Journal of Bioorganic Chemistry Volume 26 Issue 1

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