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Genetically‐encoded cyclic peptide libraries allow rapid in vivo screens for inhibitors of any target protein of interest. In particular, the Split Intein Circular Ligation of Protein and Peptides (SICLOPPS) system exploits spontaneous protein splicing of inteins to produce intracellular cyclic...
Herein, we report the synthesis of a daptomycin thiolactone analogue via the MeDbz‐linker based on resin self‐cleaving macrocyclization pathway. A detailed investigation of the reaction conditions is reported, including the variation of the cyclization buffer, temperature, concentration, and...
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