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Using monocultures or single species dominated natural phytoplankton, cell counts and volume estimations obtained by visual and electronic methods show reasonable agreement. Calibration seems possible (Figs. 1–2).
Further examples given (Fig. 3–4) show that microscopical...
A short description is given of a cell analyzer based on lightscattering properties. The apparatus has the capability to distinguish different types of cells. It is compared with a cell counter based on the Coulter principle with respect to counting. Counting results agreed very well.
In grazing experiments, the changes in fluorometric determined concentrations of chlorophyll a and phaeopigment are probably less bothered by the interference of other chlorophyll pigments than in analysis of single seawater samples. Theoretically, phaeopigment recovery will be ca 100%,...
Some specific problems of electronic particle counters (Coulter Counter, Elzone) are discussed. Conductivity of the medium does not influence the size response of the instrument, but might change the size of the particles through osmotic stress.
Different formulae used in the literature for calculation of ingestion rates are reviewed. Their applicability is discussed in relation to two criteria: the occurrence of saturation of the feeding and/or growth of the food particles. It is shown by simulations that the differences in results...
The influence of various fixatives on size distribution and cell concentration was investigated using two types of electronic particle counters. Both in autotrophic and heterotrophic flagellates drastic losses of cells and changes of size were found in all preservatives used. Also preserved...
The following scheme lists the problems which have been and will continue to be encountered in grazing experiments on natural particle assemblages. In some cases there are solutions, but many of the problems listed below remain intractable. To solve them will require innovative approaches, most...
Some of the technical problems specific to the application of radiotracer techniques for measuring grazing of zooplankton are highlighted and, in some cases, the corrective measures suggested.
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