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A cell surface display system has been developed in the yeast Yarrowia lipolytica using the cell wall protein YlPir1. The red fluorescent protein TurboFP635 was used as a model protein. The expression plasmid in which the YlPIR1 gene without stop-codon was fused in-frame with the nucleotide...
Bioactive substances (BAS) of plant origin are known to play a very important role in modern medicine. Their use, however, is often limited by availability of plant resources and may jeopardize rare species of medicinal plants. Plant cell cultures can serve as a renewable source of valuable...
The enzymes of the Entner-Doudoroff pathway (EDP) and/or the oxidative branch of the pentose phosphate pathway (OBPPP) have been inactivated in the E. coli 2Δ (ΔsucAB, ΔaceAK, P
-brnQ) [pEL-IDO] strain, which is used for the biotransformation of L-isoleucine into 4-hydroxyisoleucine (4-HIL) by...
This review is written due to an increased interest in the production of energy carriers and basic substrates of the chemical industry from renewable natural resources. In this review, the microbiological aspects of biobutanol production are reflected and the microbial producers of butanol (both...
Strains of a Stephania glabra suspension culture grown in flasks and two types of bioreactors (laboratory-scale bubble and pilot-scale stirred reactors) have been compared according to their growth characteristics and accumulation of the alkaloid stepharine. The best characteristics have been...
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