Open Advanced Search

Log in
Enterprise Plans
Sign Up

Log in
Sign Up
Enterprise Plans
Browse
Features
Pricing

DeepDyve

Advanced Search

show/hide query preview

With all of the words or DOIs:

DeepDyve's default query mode: search by keyword or DOI.

With the exact phrase:

Require these words, in this exact order. Do not surround your terms in double-quotes ("") in this field.

With at least one of the words:

Find any of these words, separated by spaces

Without the words:

Exclude each of these words, separated by spaces

With words in the article title:

Search for these terms only in the title of an article

Published between the years:

With the following authors:

Most effective as: LastName, First Name or Lastname, FN

With words in the journal name:

Search for articles published in journals where these words are in the journal name

Reset filters

Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

DeepDyve requires Javascript to function. Please enable Javascript on your browser to continue.

2D PAGE: Sample Preparation and Fractionation

Part of the Methods in Molecular Biology™ Book Series (volume 424)

Editors: Posch, Anton

DOI: 10.1007/978-1-60327-064-9

ISBN: 978-1-58829-722-8

Publisher: Humana Press - Springer Journals

Jan 1, 2008

1 - 10 of 34 Chapters

LitStream

Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization

Goldberg, Stanley

Pages: 3 –22

[This chapter covers the various methods of mechanical cell disruption and tissue homogenization that are currently commercially available for processing minute samples (<1 ml) to larger production quantities. These mechanical methods of lysing do not introduce chemicals or enzymes to the system....
LitStream

Bacteria and Yeast Cell Disruption Using Lytic Enzymes

Salazar, Oriana

Pages: 23 –34

[Enzymatic methods provide a convenient alternative for overcoming technical disadvantages of mechanical disruption. Protocols for protein extraction from bacteria and Saccharomyces cerevisiae using lytic enzymes are presented in this chapter. Adaptation of the yeast protocol to a microtiter...
LitStream

Sample Solublization Buffers for Two-Dimensional Electrophoresis

Weiss, Walter; Görg, Angelika

Pages: 35 –42

[Before two-dimensional electrophoresis (2-DE), proteins of the sample must be denatured, reduced, disaggregated, and solubilized. Sample solubilization is usually carried out in a buffer containing chaotropes (typically 9.5 M urea, or 5–8 M urea and 2 M thiourea), 2–4% nonionic and/or...
LitStream

Quantitation of Protein in Samples Prepared for 2-D Electrophoresis

Berkelman, Tom

Pages: 43 –49

[The concentration of protein in a sample prepared for two dimensional (2-D) electrophoretic analysis is usually determined by protein assay. Reasons for this include the following. (1) Protein quantitation ensures that the amount of protein to be separated is appropriate for the gel size and...
LitStream

Removal of Interfering Substances in Samples Prepared for Two-Dimensional (2-D) Electrophoresis

Berkelman, Tom

Pages: 51 –62

[Biological samples may contain contaminants that interfere with analysis by twodimensional (2-D) electrophoresis. Lysates or biological fluids are complex mixtures that contain a wide variety of nonprotein substances in addition to the proteins to be analyzed. These substances often interfere...
LitStream

Protein Concentration by Hydrophilic Interaction Chromatography Combined with Solid Phase Extraction

Schneider, Ulrich

Pages: 63 –69

[Hydrophilic interaction chromatography (HILIC) is a variant of normal phase chromatography, in which analyte molecules attach to a solid support (e.g., poly [2-hydroxyethyl] aspartamide silica) by the action of a mobile phase containing a high amount of organic modifier such as acetonitrile or...
LitStream

Difference Gel Electrophoresis Based on Lys/Cys Tagging

Westermeier, Reiner; Scheibe, Burghardt

Pages: 73 –85

[Before separation, proteins of different biological samples are labeled with different fluorescent dyes, the CyDye™ DIGE Fluors. Currently three dyes with spectrally different excitation and emission wavelengths are available. This allows labeling up to three different samples, and coseparating...
LitStream

Isotope-Coded Two-Dimensional Maps: Tagging with Deuterated Acrylamide and 2-Vinylpyridine

Righetti, Pier Giorgio; Sebastiano, Roberto; Citterio, Attilio

Pages: 87 –99

[Isotope-coded two-dimensional maps, with either D\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt}...
LitStream

Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)

Gruhler, s; Kratchmarova, Irina

Pages: 101 –111

[Quantitative proteomics has become a pivotal tool that has been applied to the investigation of many different biological processes such diverse as the detection of biomarkers in tissue samples, the regulation of cell signaling, and the characterization of protein interactions. Stable isotope...
LitStream

ICPL—Isotope-Coded Protein Label

Kellermann, Josef

Pages: 113 –123

[Stable isotope labeling in combination with mass spectrometry has emerged as a powerful tool to identify and relatively quantify thousands of proteins within complex protein mixtures. Here we describe a method, termed isotope-coded protein label (ICPL), which is capable of high-throughput...

results per page

Browse All eBooks

Features

Search and discover articles on DeepDyve, PubMed, and Google Scholar
Read the full-text of open access and premium content
Organize articles with folders and bookmarks
Collaborate on and share articles and folders

Info

Pricing
Enterprise Plans
Browse Journals & Topics
About DeepDyve

Help

Help
Publishers
Contact Us

Sign up for your DeepDyve account now!

Read and print from thousands of top scholarly journals.

System error. Please try again!

By signing up, you agree to DeepDyve’s Terms of Service and Privacy Policy.

Already have an account? Log in

Save Article to Bookmarks

Bookmark this article. You can see your Bookmarks on your DeepDyve Library.

To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.

Subscribe to Journal Email Alerts

To subscribe to email alerts, please log in first, or sign up for a DeepDyve account if you don’t already have one.

Follow a Journal

To get new article updates from a journal on your personalized homepage, please log in first, or sign up for a DeepDyve account if you don’t already have one.

Our policy towards the use of cookies

All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.

cookie policy